Tumor-derived urinary exosomal long non-coding RNAs as diagnostic biomarkers for bladder cancer

Authors

  • Maryam Abbastabar Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Mohammad Sarfi Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Abolfazl Golestani Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Ali Karimi Department of Urology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  • Gholamreza Pourmand Department of Urology, School of Medicine, Urology Research Center, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran
  • Ehsan Khalili Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran, Tel: +98 21 88953004, Fax: +98 21 64053385, E-mail: E-khalili@sina.tums.ac.ir

DOI:

https://doi.org/10.17179/excli2019-1683

Keywords:

lncRNA, exosome, bladder cancer, urine

Abstract

Bladder cancer (BC) is the sixth most common malignancy in men and 17th in women. Exosomal long non-coding RNAs (lncRNAs) have been defined as a novel biomarker for BC. The aim of this study is to evaluate the clinical significance of urine exosomal PVT-1, ANRIL and PCAT-1 as a biomarker in BC patients with tumors classified as T1 or T2. Exosomes were isolated from urine of BC patients and healthy donors, then characterized according to their shape, size, and exosome markers by Electron Microscopy, Dynamic light scattering, and Western blotting. Exosomal lncRNAs extraction was done to determine the expression levels of PVT-1, ANRIL and PCAT-1 by qRT-PCR. ANRIL and PCAT-1 expression was significantly higher in BC patients compared to normal subjects. To evaluate the performance of the identified lncRNAs for BC detection, we performed ROC curves analysis. The diagnostic accuracy of ANRIL and PCAT-1, measured by AUC, was 0.7229 (sensitivity = 46.67 % and specificity = 87.5 %) and 0.7292 (sensitivity = 43.33 % and specificity = 87.5 %). Transcript levels of lncRNAs in urinary exosomes are potential diagnostic biomarkers in bladder cancer.

Published

2020-03-04

How to Cite

Abbastabar, M., Sarfi, M., Golestani, A., Karimi, A., Pourmand, G., & Khalili, E. (2020). Tumor-derived urinary exosomal long non-coding RNAs as diagnostic biomarkers for bladder cancer. EXCLI Journal, 19, 301–310. https://doi.org/10.17179/excli2019-1683

Issue

Section

Original articles

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