Apigenin-7-O-glucoside versus apigenin: Insight into the modes of anticandidal and cytotoxic actions

Authors

  • Marija Smiljkovic Department of Plant Physiology, Institute for Biological Research "Siniša Stankovic", University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia
  • Danijela Stanisavljevic Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, 11010 Belgrade, Serbia
  • Dejan Stojkovic Department of Plant Physiology, Institute for Biological Research "Siniša Stankovic", University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia
  • Isidora Petrovic Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, 11010 Belgrade, Serbia
  • Jelena Marjanovic Vicentic Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, 11010 Belgrade, Serbia
  • Jelena Popovic Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, 11010 Belgrade, Serbia
  • Simona Golic Grdadolnik Laboratory of Biomolecular Structure, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia
  • Dejan Markovic Clinic for Pediatric and Preventative Dentistry, Faculty of Dental Medicine, Rankeova 4, Belgrade
  • Snežana Sankovic-Babice ENT Clinic, Clinical Hospital Centre Zvezdara, Presevska 31, 11000 Belgrade, Serbia
  • Jasmina Glamoclija Department of Plant Physiology, Institute for Biological Research "Siniša Stankovic", University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia
  • Milena Stevanovic Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, 11010 Belgrade, Serbia
  • Marina Sokovic Department of Plant Physiology, Institute for Biological Research "Siniša Stankovic", University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia

DOI:

https://doi.org/10.17179/excli2017-300

Keywords:

apigenin-7-O-glucoside, apigenin, antifungal, Candida spp., cytotoxic, HCT116

Abstract

Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin-7-O-glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression. Obtained results indicated considerable antifungal activity of apigenin-7-O-glucoside towards all Candida isolates. Breakdown of C. albicans plasma membrane was achieved upon treatment with apigenin-7-O-glucoside for shorter period of time then with apigenin. Reduction of intra- and extracellular reactive oxidative species was achieved with minimum inhibitory concentrations of both compounds, suggesting that reactive oxidative species inhibition could be a mechanism of antifungal action. None of the compounds exhibited binding affinity to C. albicans CYP51 protein. Besides, apigenin-7-O-glucoside was more effective compared to apigenin in reduction of cell's viability and induction of cell death of HCT116 cells. Treatment with both compounds resulted in chromatin condensation, apoptotic bodies formation and apoptotic genes expression in HCT116 cells, but the apigenin-7-O-glucoside required a lower concentration to achieve the same effect. Compounds apigenin-7-O-glucoside and apigenin displayed prominent antifungal potential and cytotoxic effect on HCT116 cells. However, our results showed that apigenin-7-O-glucoside has more potent activity compared to apigenin in all assays that we used.

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Published

2017-05-23

How to Cite

Smiljkovic, M., Stanisavljevic, D., Stojkovic, D., Petrovic, I., Marjanovic Vicentic, J., Popovic, J., … Sokovic, M. (2017). Apigenin-7-O-glucoside versus apigenin: Insight into the modes of anticandidal and cytotoxic actions. EXCLI Journal, 16, 795–807. https://doi.org/10.17179/excli2017-300

Issue

Vol. 16 (2017)

Section

Original articles

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