Ameliorative effects of high intensity interval training and Lactobacillus rhamnosus GG against tetracycline-induced fatty liver in rats

a gene expression profiling comparative study

Authors

  • Hamideh Mahmoodzadeh Hosseini Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran https://orcid.org/0000-0002-3987-0164
  • Hossein Shirvani Exercise Physiology Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Nosrati Alley, Sheikh Bahai Street, Mollasadra Street, Vanak Square, 19395-5487, Tehran, Iran. Tel: +98-21-82482395; Fax: +98-21-88600030, E-mail: shirvani@bmsu.ac.ir https://orcid.org/0000-0002-0696-958X
  • Fariba Aghaei Faculty of Physical Education and Sport Sciences, Karaj Branch, Islamic Azad University, Alborz, Iran https://orcid.org/0000-0002-5781-3454
  • Ehsan Arabzadeh Exercise Physiology Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran https://orcid.org/0000-0003-2907-9798
  • Martin Hofmeister Department of Food and Nutrition, Consumer Centre of the German Federal State of Bavaria, Munich, Germany https://orcid.org/0000-0002-0693-7887

DOI:

https://doi.org/10.17179/excli2022-4791

Keywords:

exercise training, Lactobacillus rhamnosus GG, fatty liver disease, probiotic

Abstract

Exercise training and probiotics have been suggested as a treatment for the prevention of chronic liver damage such as non-alcoholic fatty liver disease (NAFLD). Lactobacillus rhamnosus Gorbach‐Goldin (LGG) is one of the most widely used probiotic strains that decreases liver damage. Thus, this study aims to consider the ameliorative effects of high intensity interval training (HIIT) and LGG against tetracycline-induced fatty liver in rats. Eighty male Wistar rats were randomly divided into 8 groups of (n=10 each group): control, LGG, HIIT, LGG+HIIT, NAFLD, NAFLD+LGG, NAFLD+HIIT, and NAFLD+LGG+HIIT. The rats are treated by intraperitoneal injection with 140 mg/kg−1 tetracycline, an antibiotic previously known to induce steatosis. The exercise training groups performed HIIT 5 days/week for 5 weeks. 107 colony-forming units (cfu) of LGG were gavaged for LGG groups 5 days/week for 5 weeks. Probiotic supplementation in combination with interval training significantly decreased tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) mRNA and matrix metalloproteinase-2 (MMP-2) mRNA in the liver (p<0.05), while the levels of lysosomal acid lipase (LIPA) mRNA was significantly increased compared to NAFLD group. Also, compared with NAFLD group, NAFLD+LGG, NAFLD+HIIT and NAFLD+LGG+HIIT groups showed a significant decrease in hepatic monocyte chemoattractant protein-1 (MCP-1). Compared to LGG and LGG+HIIT groups, all NAFLD groups showed a significant decrease in apolipoprotein C3 (APOC3) in liver tissue (p<0.05). The results suggested that interval exercise with LGG supplementation minimizes cell destruction and inflammation in liver tissue due to NAFLD by improving gene expression profiles.

Author Biographies

Hamideh Mahmoodzadeh Hosseini, Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

Professor (Assistant)

Hossein Shirvani, Exercise Physiology Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Nosrati Alley, Sheikh Bahai Street, Mollasadra Street, Vanak Square, 19395-5487, Tehran, Iran. Tel: +98-21-82482395; Fax: +98-21-88600030, E-mail: shirvani@bmsu.ac.ir

Associate Professor

Fariba Aghaei, Faculty of Physical Education and Sport Sciences, Karaj Branch, Islamic Azad University, Alborz, Iran

Assistant Professor

Ehsan Arabzadeh, Exercise Physiology Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

Assistant Professor

Martin Hofmeister, Department of Food and Nutrition, Consumer Centre of the German Federal State of Bavaria, Munich, Germany

PhD

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Published

2022-07-21

How to Cite

Hosseini, H. M., Shirvani, H., Aghaei, F., Arabzadeh, E., & Hofmeister, M. (2022). Ameliorative effects of high intensity interval training and Lactobacillus rhamnosus GG against tetracycline-induced fatty liver in rats: a gene expression profiling comparative study. EXCLI Journal, 21, 991–1006. https://doi.org/10.17179/excli2022-4791

Issue

Vol. 21 (2022)

Section

Original articles

Categories

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Copyright (c) 2022 Hamideh Mahmoodzadeh Hosseini, Hossein Shirvani, Fariba Aghaei, Ehsan Arabzadeh, Martin Hofmeister

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