Trans-1,3-diphenyl-2,3-epoxypropan-1-one, a chalcone derivative, induces apoptosis via ROS-mediated down-regulation of Bcl-xL in human leukemia HL-60 cells

Authors

  • Eun-Yi Ko Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea; School of Marine Biomedical Sciences, Jeju National University, Jeju 690-756, Republic of Korea
  • Seung-Hong Lee Division of Food Bioscience, Konkuk University, Chungju Chungbuk 380-701, Republic of Korea
  • Ji-Yeon Ko School of Marine Biomedical Sciences, Jeju National University, Jeju 690-756, Republic of Korea
  • Jeong Yong Moon Subtropical Horticulture Research Institute, Jeju National University, Jeju 690-756, Republic of Korea
  • Weon-Jong Yoon Jeju Biodiversity Research Institute, Jeju Technopark, Jeju, 699-943, Republic of Korea
  • Ginnae Ahn Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 550-749, Republic of Korea
  • Seong Woon Roh Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea
  • Kichul Cho Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea
  • You-Jin Jeon School of Marine Biomedical Sciences, Jeju National University, Jeju 690-756, Republic of Korea
  • Daekyung Kim Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea
  • Kil-Nam Kim Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea; Department of Marin Biotechnology, University of Science and Technology, Daejeon 305-350, Republic of Korea

DOI:

https://doi.org/10.17179/excli2015-373

Keywords:

anticancer, trans-1,3-diphenyl-2,3-epoxypropan-1-one (DPEP), apoptosis, reactive oxygen species (ROS), Bcl-xL

Abstract

The anticancer effects of trans-1,3-diphenyl-2,3-epoxypropan-1-one (DPEP), a chalcone derivative, were investigated in human leukemia HL-60 cells. Treatment of HL-60 cells with various concentration of DPEP resulted in a sequence of events characteristic of apoptosis, including loss of cell viability, morphological changes, and increased sub-G1 DNA content. We demonstrated that DPEP elevates reactive oxygen species (ROS) levels in HL-60 cells, and that the ROS scavenger N-acetylcysteine (NAC) could block DPEP-induced ROS generation and apoptosis. Western blot analysis revealed that DPEP inhibits Bcl-xL expression, leading to caspase-3 activation and poly-ADP-ribose polymerase (PARP) cleavage, thereby inducing apoptosis. However, NAC pre-treatment significantly inhibited the activation of caspase-3 and PARP cleavage and reduced Bcl-xL levels. These findings provide the first evidence that DPEP may inhibit the growth of HL-60 cells and induce apoptosis through a ROS-mediated Bcl-xL pathway.

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Published

2015-08-03

How to Cite

Ko, E.-Y., Lee, S.-H., Ko, J.-Y., Moon, J. Y., Yoon, W.-J., Ahn, G., … Kim, K.-N. (2015). Trans-1,3-diphenyl-2,3-epoxypropan-1-one, a chalcone derivative, induces apoptosis via ROS-mediated down-regulation of Bcl-xL in human leukemia HL-60 cells. EXCLI Journal, 14, 900–907. https://doi.org/10.17179/excli2015-373

Issue

Vol. 14 (2015)

Section

Original articles

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