Antibacterial and cytotoxic activities of naphthoquinone pigments from Onosma visianii Clem

Authors

  • Milena D. Vukic University of Kragujevac, Faculty of Science, Department of Chemistry, R. Domanovica 12, 34000 Kragujevac, Serbia
  • Nenad L. Vukovic University of Kragujevac, Faculty of Science, Department of Chemistry, R. Domanovica 12, 34000 Kragujevac, Serbia
  • Gorica T. Djelic University of Kragujevac, Faculty of Science, Department of Biology and Ecology, R. Domanovica 12, 34000 Kragujevac, Serbia
  • Suzana Lj. Popovic University of Kragujevac, Faculty of Medical Sciences, Centre for Molecular Medicine and Stem Cell Research, 34000 Kragujevac, Serbia
  • Milan M. Zaric University of Kragujevac, Faculty of Medical Sciences, Department of Biochemistry, 34000 Kragujevac, Serbia
  • Dejan D. Baskic University of Kragujevac, Faculty of Medical Sciences, Centre for Molecular Medicine and Stem Cell Research, 34000 Kragujevac, Serbia
  • Gordana B. Krstic University of Belgrade, Faculty of Chemistry, Belgrade, Serbia
  • Vele V. Tesevic University of Belgrade, Faculty of Chemistry, Belgrade, Serbia
  • Miroslava M. Kacaniova University of Agriculture in Nitra, Department of Microbiology, Faculty of Biotechnology and Food Science, Slovak Nitra, Slovakia

DOI:

https://doi.org/10.17179/Excli2016-762

Keywords:

Onosma visianii, naphthoquinones, antibacterial activity, cytotoxic activity

Abstract

In this study, the antibacterial and cytotoxic activities of isolated compounds from the roots of Onosma visianii were investigated. By using different chromatographic techniques and appropriate spectroscopic methods, the seven naphthoquinones were described: deoxyshikonin (1), isobutyrylshikonin (2), α-methylbutyrylshikonin (3), acetylshikonin (4), β-hydroxyisovalerylshikonin (5), 5,8-O-dimethyl isobutyrylshikonin (6) and 5,8-O-dimethyl deoxyshikonin (7). Among the tested compounds, 3 and 4 exhibited the highest antibacterial activities toward all tested bacterial species (MIC50 and MIC90 for gram positive bacteria: 6.40 μg/mL-12.79 μg/mL and 6.82 μg/mL-13.60 μg/mL, respectively; for gram negative bacteria: 4.27 μg/mL-8.53 μg/mL and 4.77 μg/mL-9.54 μg/mL, respectively). Also, naphthoquinones 3 and 4 exhibited strong cytotoxic activity against MDA-MB-231 cells (IC50 values 86.0 μg/mL and 80.2 μg/mL, respectively), while compounds 1, 3, 4 and 5 significantly decreased viability of HCT116 cells (IC50 values of 97.8 μg/mL, 15.2 μg/mL, 24.6 μg/mL and 30.9 μg/mL, respectively). Our results indicated that all tested naphthoquinone pigments are potential candidates for clinical uses as antibacterial and cytotoxic agents.

Published

2017-02-16

How to Cite

Vukic, M. D., Vukovic, N. L., Djelic, G. T., Popovic, S. L., Zaric, M. M., Baskic, D. D., … Kacaniova, M. M. (2017). Antibacterial and cytotoxic activities of naphthoquinone pigments from Onosma visianii Clem. EXCLI Journal, 16, 73–88. https://doi.org/10.17179/Excli2016-762

Issue

Section

Original articles

Most read articles by the same author(s)