Seasonal variability of Chelidonium majus L. secondary metabolites content and antioxidant activity

Authors

  • Z. Dragana Jakovljevic Department of Biology and Ecology, Faculty of Science, University of Kragujevac,str. Radoja Domanovica No. 12, 34000 Kragujevac, Republic of Serbia
  • S. Milan Stankovic Department of Biology and Ecology, Faculty of Science, University of Kragujevac,str. Radoja Domanovica No. 12, 34000 Kragujevac, Republic of Serbia
  • D. Marina Topuzovic Department of Biology and Ecology, Faculty of Science, University of Kragujevac,str. Radoja Domanovica No. 12, 34000 Kragujevac, Republic of Serbia

Keywords:

Ch. majus, seasonal variation, antioxidant activity, phenols, flavonoids

Abstract

The aim of this study is to investigate the total phenolic content, concentration of flavonoids and antioxidant activity in extracts of the plant Chelidonium majus L. during different phenological stages (stage of rosette, the initial flowering stage, the stage of fully formed flowers and stage of fruits formation). Five different extracts of the whole plant, for each phase, were obtained by extraction with water, methanol, acetone, ethyl acetate and petroleum ether. The concentration of total phenolic content was determined using the Folin-Ciocalteu´s reagent and obtained values were the highest in the rosette stage (60.96 mg GA/g). The concentration of flavonoids was the highest in the initial stage of flowering (291.58 mg RU/g). The antioxidant activity was determined in vitro using DPPH reagent. The highest antioxidant activity was expressed in the rosette stage (50.72 mg/ml). Based on the obtained results it can be concluded that the concentrations of secondary metabolites in Ch. majus depend on the phenological stage of the plant.

Published

2013-03-15

How to Cite

Jakovljevic, Z. D., Stankovic, S. M., & Topuzovic, D. M. (2013). Seasonal variability of Chelidonium majus L. secondary metabolites content and antioxidant activity. EXCLI Journal, 12, 260–268. Retrieved from https://www.excli.de/index.php/excli/article/view/1147

Issue

Section

Original articles