Three dimensional electron microscopy and in silico tools for macromolecular structure determination

Authors

  • Subhomoi Borkotoky Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry-605014, India
  • Chetan Kumar Meena Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry-605014, India
  • Mohammad Wahab Khan Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry-605014, India
  • Ayaluru Murali Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry-605014, India

Keywords:

cryo-EM, single particle analysis, CTF correction, segmentation

Abstract

Recently, structural biology witnessed a major tool - electron microscopy - in solving the structures of macromolecules in addition to the conventional techniques, X-ray crystallography and nuclear magnetic resonance (NMR). Three dimensional transmission electron microscopy (3DTEM) is one of the most sophisticated techniques for structure determination of molecular machines. Known to give the 3-dimensional structures in its native form with literally no upper limit on size of the macromolecule, this tool does not need the crystallization of the protein. Combining the 3DTEM data with in silico tools, one can have better refined structure of a desired complex. In this review we are discussing about the recent advancements in three dimensional electron microscopy and tools associated with it.

Published

2013-04-24

How to Cite

Borkotoky, S., Meena, C. K., Khan, M. W., & Murali, A. (2013). Three dimensional electron microscopy and in silico tools for macromolecular structure determination. EXCLI Journal, 12, 335–346. Retrieved from https://www.excli.de/index.php/excli/article/view/1152

Issue

Section

Review articles