Total phenolic, antioxidant, antimicrobial activities and toxicity study of Gynotroches axillaris blume (Rhizophoraceae)

Authors

  • Salam Ahmed Abed Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 Skudai, Johor
  • Hasnah Mohd Sirat Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 Skudai, Johor
  • Muhammad Taher Department of Pharmaceutical Technology, Faculty of Pharmacy, Universiti Islam Antarabangsa Malaysia, Kuantan, Pahang

Keywords:

Gynotroches axillaris, Rhizophoraceae, total phenolic, antioxidant, antimicrobial, toxicity

Abstract

The antioxidant activity and the total phenolic content, as well as the influence of petroleum ether, chloroform and methanol extracts from the leaves of Gynotroches axillaris, on microorganisms were studied. The total phenolic contents were evaluated by using Folin-Ciocalteu reagent and the obtained values ranged from 70.0 to 620 mg GAE/g. The efficiency of antioxidation, which was identified through the scavenging of free radical DPPH, exhibited that the highest IC50 was in the methanolic extract (44.7 µg/mL) as compared to the standard ascorbic acid (25.83 µg/mL) and to standard BHT (17.2 µg/mL). In vitro antimicrobial activity of extracts was tested against Gram-negative bacteria, Gram-positive bacteria and fungi. Methanol extract showed activity in the range (225-900 μg/mL) with both types, while petroleum ether and chloroform extracts were only active with Bacillus subtilis. The three extracts strongly inhibited all fungi with activity 225-450 μg/mL. The toxicity test against brine shrimps indicated that all extracts were non-toxic with LC50 value more than 1000 µg/mL. The finding of this study supports the safety of these extracts to be used in medical treatments.

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Published

2013-05-13

How to Cite

Abed, S. A., Sirat, H. M., & Taher, M. (2013). Total phenolic, antioxidant, antimicrobial activities and toxicity study of Gynotroches axillaris blume (Rhizophoraceae) . EXCLI Journal, 12, 404–412. Retrieved from https://www.excli.de/index.php/excli/article/view/1158

Issue

Vol. 12 (2013)

Section

Original articles

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