Production, purification and characterization of novel beta glucosidase from newly isolated Penicillium simplicissimum H-11 in submerged fermentation

Authors

  • Hongzhi Bai School of Land and environment, Shenyang Agricultural University, Shenyang 110866 Liaoning, P.R. China
  • Hui Wang Bioscience and Biotechnology College, Shenyang Agricultural University, Shenyang 110866 Liaoning, P.R. China
  • Junde Sun School of Land and environment, Shenyang Agricultural University, Shenyang 110866 Liaoning, P.R. China
  • Muhammad Irfan Bioscience and Biotechnology College, Shenyang Agricultural University, Shenyang 110866 Liaoning, P.R. China
  • Mei Han School of Land and environment, Shenyang Agricultural University, Shenyang 110866 Liaoning, P.R. China
  • Yuqian Huang School of Land and environment, Shenyang Agricultural University, Shenyang 110866 Liaoning, P.R. China
  • Xiaori Han School of Land and environment, Shenyang Agricultural University, Shenyang 110866 Liaoning, P.R. China
  • Qian Yang Center of Life Science and Technology Harbin Institute of Technology Harbin, China

Keywords:

beta-glucosidase, Penicillium simplicissimum, purification, characterization

Abstract

β-Glucosidase is an important component of the cellulase complex. It not only hydrolyzes cellobiose and short-chain cellooligosaccharides to glucose, but also removes the inhibitory effect of cellobiose on the β-1, 4-endoglucanase and exoglucanase, thereby increasing the overall rate of cellulose biodegradation. β-glucosidasefrom culture supernatant of a fungus Penicillium simplicissimum was purified to homogeneity, by using ammonium sulfate fraction, Sephadex G-100 chromatography, and its properties were studied. The molecular mass of the enzyme was about 126.0 kDa, as identified by 12% SDS-PAGE. The optimum pH and temperature were 4.4 ~ 5.2 and 60 °C, respectively. The enzyme was stable in pH 5.2 ~ 6.4 and under 40 °C. Metal profile of the enzyme showed that Mn2+ enhances its activity, while Cu2+, Co2+and Fe3+ cause obvious inhibition. The Km and Vmax was 14.881 mg/ml and 0.364 mg ml/min against salicin as a Substrate. This enzyme had secondary protein structure as evidenced by FTIR spectrum.

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Published

2013-06-13

How to Cite

Bai, H., Wang, H., Sun, J., Irfan, M., Han, M., Huang, Y., … Yang, Q. (2013). Production, purification and characterization of novel beta glucosidase from newly isolated Penicillium simplicissimum H-11 in submerged fermentation. EXCLI Journal, 12, 528–540. Retrieved from https://www.excli.de/index.php/excli/article/view/1170

Issue

Vol. 12 (2013)

Section

Original articles

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