Sorption of DNA by diatomite-Zn(II) embedded supermacroporous monolithic p(HEMA) cryogels

Authors

  • Kabil Özcan Tozak Aksaray University, Faculty of Art and Science, Chemistry Department, 68100 Aksaray, Turkey
  • Mahmut Erzengin Aksaray University, Faculty of Art and Science, Chemistry Department, 68100 Aksaray, Turkey
  • Idris Sargin Aksaray University, Faculty of Art and Science, Chemistry Department, 68100 Aksaray, Turkey
  • Nuri Ünlü Aksaray University, Faculty of Art and Science, Chemistry Department, 68100 Aksaray, Turkey

Keywords:

diatomite, particle embedding, composite cryogel, immobilized metal affinity chromatography

Abstract

In this study, the DNA sorption performance of diatomite-Zn(II) embedded supermacroporous monolithic p(HEMA) cryogels were investigated for the purpose of designing a novel adsorbent that can be utilized for DNA purification, separation and immunoadsorption studies such as removal of anti-dsDNA antibodies from systemic lupus erythematosus (SLE) patient plasma. Poly(2-hydroxyethyl methacrylate) [p(HEMA)]-based monolithic cryogel column embedded with Zn2+-diatomite particles was prepared by free radical cryo-copolymerization of 2-hydroxyethyl methacrylate (HEMA) with N,N'-methylene-bis-acrylamide (MBAAm). The polymerization reaction was initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. After thawing, the monolithic composite cryogels were used for affinity sorption and then subsequent desorption of DNA molecules from aqueous solutions. Diatomite (DA) particles were characterized by XRF and BET method. The characterization of composite cryogel was done through SEM imaging. The effects of pH of the solution, initial DNA concentration, ionic strength, temperature and flow rates on adsorption were investigated to determine the optimum conditions for adsorption/desorption experiments. The particle embedding procedure was shown to yield significantly enhanced adsorption of DNA on the adsorbent. Furthermore, considering its excellent bio-compatibility, p(HEMA) cryogels are promising a candidate for further DNA sorption studies.

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Published

2013-08-12

How to Cite

Tozak, K. Özcan, Erzengin, M., Sargin, I., & Ünlü, N. (2013). Sorption of DNA by diatomite-Zn(II) embedded supermacroporous monolithic p(HEMA) cryogels. EXCLI Journal, 12, 670–680. Retrieved from https://www.excli.de/index.php/excli/article/view/1184

Issue

Vol. 12 (2013)

Section

Original articles

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