High level of miR-196b at newly diagnosed pediatric acute myeloid leukemia predicts a poor outcome

Main Article Content

Lihua Xu
Yang Guo
Wenying Yan
Jiannong Cen
Yuna Niu
Qing Yan
Hailong He
Chien-Shing Chen
Shaoyan Hu

Abstract

Differential expression of microRNAs (miRNAs) has been implicated in leukemogenesis. We investigate the expression pattern of miR-196b. Using quantitative real-time PCR (qRT-PCR), we detected the expression of miR-196b and its correlated genes (SMC1A/MLH1) in initial pediatric AML. A significant association was observed between overexpression of miR-196b and inferior overall survival of pediatric AML (Log Rank P<0.0001). AML M4/5 subtype, high white blood cell (WBC) count at presentation, MLL rearrangement, or FLT3-ITD mutation at diagnosis and non-remission group after the first induction chemotherapy possessed higher miR-196b expression. Furthermore, a positive relationship was found between the expression of miR-196b and SMC1A/MLH1 (Spearman's r=0.37 and 0.44, P=0.001 and <0.0001, respectively). Taken together, these findings suggest that differentially high expression of miR-196b in diagnostic marrow samples of pediatric AML is associated with unfavorable outcome, and miR-196b potentially can be a novel biomarker for the diagnosis, prognosis and treatment in pediatric AML.

Article Details

How to Cite
Xu, L., Guo, Y., Yan, W., Cen, J., Niu, Y., Yan, Q., He, H., Chen, C.-S., & Hu, S. (2017). High level of miR-196b at newly diagnosed pediatric acute myeloid leukemia predicts a poor outcome. EXCLI Journal, 16, 197-209. https://doi.org/10.17179/excli2016-707
Section
Original articles