Heme oxygenase effect on mesenchymal stem cells action on experimental Alzheimer's disease

Authors

  • MT Abdel Aziza Department of Medical Biochemistry, Unit of Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt
  • HM Atta Clinical Biochemistry Department, King Abdulaziz University, Jeddah, Saudi Arabia
  • H Samer Department of Neurology, Faculty of Medicine, Cairo University, Cairo, Egypt
  • HH Ahmed Department of Medical Biochemistry, Unit of Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt
  • LA Rashed Department of Medical Biochemistry, Unit of Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt
  • D Sabry Department of Medical Biochemistry, Unit of Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt
  • ER Abdel Raouf Department of Pediatrics, National Research Center
  • Marwa Abdul latif Alkaffas Department of Medical Biochemistry, Unit of Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt

Keywords:

Alzheimer's disease, stem cell, heme oxygenase

Abstract

The objective is to evaluate the effect of heme oxygenase-1 (HO-1) enzyme inducer and inhibitor on Mesenchymal Stem Cells (MSCs) in Alzheimer disease. 70 female albino rats were divided equally into 7 groups as follows: group 1: healthy control; group 2: Aluminium chloride induced Alzheimer disease; group 3: induced Alzheimer rats that received intravenous injection of MSCs; group 4: induced Alzheimer rats that received MSCs and HO inducer cobalt protoporphyrin; group 5: induced Alzheimer rats that received MSCs and HO inhibitor zinc protoporphyrin; group 6: induced Alzheimer rats that received HO inducer; group7: induced Alzheimer rats that received HO inhibitor. Brain tissue was collected for HO-1, seladin-1 gene expression by real time polymerase chain reaction, heme oxygenase activity, cholesterol estimation and histopathological examination. MSCs decreased the plaque lesions, heme oxygenase induction with stem cells also decreased plaque lesions however there was hemorrhage in the brain. Both heme oxygenase inducer alone or with stem cells increased seladin-1 expression and decreased cholesterol level. MSCs alone or with HO-1 induction exert a therapeutic effect against the brain lesion in Alzheimer's disease possibly through decreasing the brain cholesterol level and increasing seladin-1 gene expression.

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Published

2013-09-03

How to Cite

Abdel Aziza, M., Atta, H., Samer, H., Ahmed, H., Rashed, L., Sabry, D., … Alkaffas, M. A. latif. (2013). Heme oxygenase effect on mesenchymal stem cells action on experimental Alzheimer’s disease. EXCLI Journal, 12, 778–792. Retrieved from https://www.excli.de/index.php/excli/article/view/1192

Issue

Vol. 12 (2013)

Section

Original articles

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