DNA and chromosomal damage in coronary artery disease patients

Authors

  • Mohd Akbar Bhat Department of Human Genetics, Guru Nanak Dev University Amritsar 143005
  • Naresh Mahajan Department of Human Genetics, Guru Nanak Dev University Amritsar 143005
  • Gursatej Gandhi Department of Human Genetics, Guru Nanak Dev University Amritsar 143005

Keywords:

coronary artery disease, DNA damage, chromosomal damage, comet assay, CBMN assay

Abstract

DNA and chromosomal damage in peripheral blood leukocytes of patients with coronary artery disease (CAD) were investigated by using the single cell gel electrophoresis assay /comet and cytokinesis- block micronucleus (CBMN) assays, respectively. The case-control study comprised patients with CAD (n = 46; average age 53.0 ± 1.27 y) undergoing treatment at local hospitals, and healthy age-and sex-matched controls (n = 19; average age 54.21 ± 0.91 y) from the general population. The results of the comet assay revealed that the mean values of DNA damage were significantly (p < 0.001) higher in CAD patients than in controls (Tail DNA% 11.55 ± 0.38 vs. 5.31 ± 0.44; Tail moment 6.17 ± 0.31 vs. 2.93 ± 0.21 AU; Olive tail moment 3.52 ± 0.23 vs. 1.25 ± 0.11 AU). The mean values of chromosomal damage were also significantly higher (p < 0.001) in CAD patients than in controls (Binucleated cells with MN- 28.15 ± 1.18 vs. 18.16 ± 2.59; micronuclei 29.52 ± 1.21 vs. 18.68 ± 2.64, respectively) while nuclear division index (1.48 ± 0.01 vs. 1.63 ± 0.01) was significantly higher (p < 0.001) in controls. The results of the present study indicate that coronary artery disease patients had increased levels of both, unrepaired (DNA) and repaired (chromosomal) genetic damage which may be a pathological consequence of the disease and/or the drug-treatment. This accumulation of DNA/chromosomal damage is of concern as it can lead to the development of cancer with increased chances of morbidity and mortality in the CAD patients.

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Published

2013-10-08

How to Cite

Bhat, M. A., Mahajan, N., & Gandhi, G. (2013). DNA and chromosomal damage in coronary artery disease patients. EXCLI Journal, 12, 872–884. Retrieved from https://www.excli.de/index.php/excli/article/view/1198

Issue

Vol. 12 (2013)

Section

Original articles

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