Enzymatic hydrolysate from velvet antler suppresses adipogenesis in 3T3-L1 cells and attenuates obesity in high-fat diet-fed mice

Authors

  • Yuling Ding Department of Animal Bio and Applied Chemistry, Konkuk University, Chungju, 27478, Republic of Korea
  • Yanmei Wang Jilin Sino-Rok Institue of Animal Science, Changchun, 130-600, China
  • Byong-Tae Jeon Division of Food Bioscience and Korea Nokyong Research Center, Konkuk University, Chungju, 27478, Republic of Korea
  • Sang-Ho Moon Division of Food Bioscience and Korea Nokyong Research Center, Konkuk University, Chungju, 27478, Republic of Korea
  • Seung-Hong Lee Division of Food Bioscience and Korea Nokyong Research Center, Konkuk University, Chungju, 27478, Republic of Korea

DOI:

https://doi.org/10.17179/excli2016-638

Keywords:

velvet antler, enzymatic hydrolysate, 3T3-L1 cells, high-fat diet, anti-obesity effect

Abstract

The purpose of the current study was to investigate the potential anti-obesity activity of an enzymatic hydrolysate of velvet antler in inhibiting adipogenesis in 3T3-L1 cells and in high-fat diet (HFD)-fed obese mice. The enzymatic hydrolysate was prepared using the commercial food grade protease, Protamex. The velvet antler Protamex hydrolysate (VAPH) indicated profound inhibitory effects on adipogenesis dose-dependently by decreasing the accumulation of triglycerides and down-regulating expression levels of adipogenesis-related proteins C/EBPα, SREBP-1, and PPARγ. In a mouse model of HFD-induced obesity, oral administration of VAPH (100 and 300 mg/kg for 13 weeks) significantly reduced the body weight gain that had resulted from the HFD. VAPH treatment also lowered the serum glucose and triglyceride levels, while increasing the HDL-C level. Furthermore, the treatment greatly reduced hepatic lipid droplet accumulation as well as the size of adipocytes. Current findings demonstrate that VAPH has profound anti-obesity effects and could be an effective candidate for preventing obesity and obesity-related chronic diseases.

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Published

2017-03-22

How to Cite

Ding, Y., Wang, Y., Jeon, B.-T., Moon, S.-H., & Lee, S.-H. (2017). Enzymatic hydrolysate from velvet antler suppresses adipogenesis in 3T3-L1 cells and attenuates obesity in high-fat diet-fed mice. EXCLI Journal, 16, 328–339. https://doi.org/10.17179/excli2016-638

Issue

Vol. 16 (2017)

Section

Original articles

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