Δ-Aminolevulinate dehydratase and glutathione peroxidase activity in Alzheimer's disease: a case-control study

Authors

  • Quelen Iane Garlet Departamento de Farmacologia, Instituto de Ciências Biológicas, Universidade Federal de Rio Grande, Rio Grande/RS, Brazil
  • Maria Vaitsa Losh Haskel Departamento de Fisiologia Humana, Universidade Federal do Rio Grande do Sul, Porto Alegre/RS90040-060, Brazil
  • Romaiana Picada Pereira Departamento de Química, Universidade Estadual de Ponta Grossa, Ponta Grossa/PR, Brazil
  • Weber Cláudio Francisco Nunes da Silva Universidade Estadual do Centro-Oeste, Campus CEDETEG, Departamento de Farmácia, Guarapuava/PR, Brazil
  • João B. Teixeira da Rocha Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria/RS, Brazil
  • Cláudia Sirlene Oliveira Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria/RS, Brazil; Programa Pós-Graduação Stricto Sensu em Biotecnologia Aplicada a Saúde da Criança e do Adolescente, Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba/PR, Brazil; Faculdades Pequeno Príncipe, Curitiba/PR, Brazil; E-mail: claudia.bioquimica@yahoo.com.br
  • Juliana Sartori Bonini Universidade Estadual do Centro-Oeste, Campus CEDETEG, Departamento de Farmácia, Guarapuava/PR, Brazil; E-mail: juliana.bonini@gmail.com

DOI:

https://doi.org/10.17179/excli2019-1749

Keywords:

Alzheimer’s disease, δ-ALA-D, Gpx, AD marker, CDR, MMSE

Abstract

Alzheimer’s disease (AD) is a neurodegenerative pathology that affects elderly people all over the world. Several studies have demonstrated that oxidative stress is an aggravating factor for AD development and progression. Therefore, this study aimed to evaluate the activity of two oxidative stress markers, glutathione peroxidase (GPx) and δ-aminolevulinate dehydratase (δ-ALA-D), as well as correlate them with blood metal levels and AD progression. For this purpose, 88 elderly individuals were divided in two groups: AD group (34 patients diagnosed with AD) and control group (34 subjects paired by age with the AD group). The Mini-Mental State Examination and the Clinical Dementia Rating (CDR) were used as tools to classify the AD progression. GPx and δ-ALA-D activities were measured in all subjects through blood tests. Both enzymes’ activities were decreased in AD patients when compared to the age-matched control group, regardless of the CDR. Moreover, GPx activity was positively correlated with selenium levels in the blood; and the δ-ALA-D activity was negatively correlated with blood copper levels. Taken together, our results indicated that, for the first time, blood δ-ALA-D activity was significantly inhibited in AD patients. While literature reports conflicting data regarding GPx activity in AD patients, the δ-ALA-D activity seems to be a more consistent tool to be applied as an earlier AD marker.

Published

2019-09-25

How to Cite

Garlet, Q. I., Haskel, M. V. L., Pereira, R. P., da Silva, W. C. F. N., da Rocha, J. B. T., Oliveira, C. S., & Bonini, J. S. (2019). Δ-Aminolevulinate dehydratase and glutathione peroxidase activity in Alzheimer’s disease: a case-control study. EXCLI Journal, 18, 866–875. https://doi.org/10.17179/excli2019-1749

Issue

Section

Original articles