Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest

Authors

  • Maheshwari Rajamanikyam Biology Division, CSIR-Indian Institute of Chemical Technology (IICT), Uppal Road, Tarnaka, Hyderabad -500 007, India
  • Varahalarao Vadlapudi Biology Division, CSIR-Indian Institute of Chemical Technology (IICT), Uppal Road, Tarnaka, Hyderabad -500 007, India
  • Sai Prathima Parvathaneni Crop Protection Chemicals Division, CSIR-Indian Institute of Chemical Technology, Uppal Road, Tarnaka, Hyderabad -500 007, India
  • Dhevendar Koude Biology Division, CSIR-Indian Institute of Chemical Technology (IICT), Uppal Road, Tarnaka, Hyderabad -500 007, India
  • Prabhakar Sripadi National Centre for Mass Spectrometry, CSIR-Indian Institute of Chemical Technology, Uppal Road, Tarnaka, Hyderabad -500 007, India
  • Sunil Misra Biology Division, CSIR-Indian Institute of Chemical Technology (IICT), Uppal Road, Tarnaka, Hyderabad -500 007, India
  • Ramars Amanchy Biology Division, CSIR-Indian Institute of Chemical Technology (IICT), Uppal Road, Tarnaka, Hyderabad -500 007, India
  • Suryanarayana Murty Upadhyayula Biology Division, CSIR-Indian Institute of Chemical Technology (IICT), Uppal Road, Tarnaka, Hyderabad -500 007, India; NIPER Guwahati, 1st Floor, Institute of Pharmacy, Guwahati Medical College & Hospital Guwahati -781 032, India

DOI:

https://doi.org/10.17179/excli2017-145

Keywords:

Brevibacterium mcbrellneri, di-(2-ethylhexyl) phthalate, dibutyl phthalate, anti-bacterial, Mosquito larvicidal, cytotoxicity, cell cycle arrest

Abstract

Bacteria belonging to the family Brevibacterieae are ubiquitous Gram positive organisms that are responsible for the feet odour and cheese aroma. Brevibacterium mcbrellneri is a relatively new member belonging to Brevibacterieae. In the current manuscript we discuss isolation of biologically active metabolites from Brevibacterium mcbrellneri. Two aromatic esters were isolated from Brevibacterium mcbrellneri by “Bioassay guided fractionation strategy” and identified as di-(2-ethylhexyl) phthalate and dibutyl phthalate by chemical characterization using biophysical techniques. The phthalate compounds show broad spectrum antibacterial activity and mosquito larvicidal activity. Mosquito larvicidal activity has been attributed to inhibition of acetylcholinesterase enzyme activity. These compounds were found to be cytotoxic in multiple cell lines causing cell cycle arrest in G1 phase.

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Published

2017-03-24

How to Cite

Rajamanikyam, M., Vadlapudi, V., Parvathaneni, S. P., Koude, D., Sripadi, P., Misra, S., … Upadhyayula, S. M. (2017). Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest. EXCLI Journal, 16, 375–387. https://doi.org/10.17179/excli2017-145

Issue

Vol. 16 (2017)

Section

Original articles

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