Involvement of haptoglobin phenotypes and genotypes in non-muscle invasive bladder cancer: A possible prognostic marker for risk stratification

Authors

  • Nazi Aghaalikhani Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  • Mojtaba Zamani Department of Agronomy and Plant Breeding, School of Agriculture, University of Tehran, Karaj, Iran
  • Abdolamir Allameh Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  • Amir Mashayekhi Department of Immunology, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran
  • Pejman Shadpour Hasheminejad Kidney Center (HKC), Hospital Management Research Center (HMRC), Iran University of Medical Sciences (IUMS), Tehran, Iran
  • Marzieh Mahmoodi Faculty of Health and Nutrition, Bushehr University of Medical Sciences, Bushehr, Iran
  • Nadereh Rashtchizadeh Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Tel: +98 41 3364666; Fax: +98 41 34426078; E-mail: rashtchizadeh@rocketmail.com

DOI:

https://doi.org/10.17179/excli2019-1768

Keywords:

Haptoglobin, Acute-phase protein, Biomarkers, Non-muscle invasive bladder cancer, Electrophoresis

Abstract

The association of haptoglobin (Hp) with various cancers has been reported and also it has been documented that the Hp phenotypes/genotypes have different functional ability. So, we examined phenotypes/genotypes of Hp in newly diagnosed, untreated non-muscle invasive bladder cancer (NMIBC) patients and investigated its prognostic value for risk stratification of the cancer. In eighty NMIBC patients and 80 healthy individuals the Hp genotypes and phenotypes were analyzed using polymerase chain reaction (PCR) and two-dimensional gel electrophoresis (2D-GE), respectively. Besides, the presence of the Hpα1, α2, and β chains in the sera was confirmed by Mass Spectrometry (MS). The frequencies of the 1-1 and 2-2 genotypes/phenotypes were respectively higher and lower in healthy subjects compared to the patients. Our results revealed that the 2-2 genotype/phenotype could increase the risk of NMIBC. There was a positive association between the 2-2 genotype/phenotype with the T category/grade of cancer (p<0.05). The present study implied a strong association between the Hp phenotypes and genotypes with NMIBC. It was found that the 2-2 genotype and phenotype could be a risk factor for NMIBC incidence, as well as, progression. This study introduced Hp genotyping as a possible cost-effective and precise method for prognosis of individuals at the risk of NMIBC.

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Published

2020-03-10

How to Cite

Aghaalikhani, N., Zamani, M., Allameh, A., Mashayekhi, A., Shadpour, P., Mahmoodi, M., & Rashtchizadeh, N. (2020). Involvement of haptoglobin phenotypes and genotypes in non-muscle invasive bladder cancer: A possible prognostic marker for risk stratification. EXCLI Journal, 19, 351–359. https://doi.org/10.17179/excli2019-1768

Issue

Vol. 19 (2020)

Section

Original articles

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