Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line

Authors

  • Mohammad Ahmad Herbal Bioactive Research Laboratory, Faculty of Pharmacy, Integral University, Lucknow, India
  • - Sahabjada Molecular Endocrinology Laboratory, Department of Zoology, University of Lucknow, Lucknow, India
  • Juber Akhtar Herbal Bioactive Research Laboratory, Faculty of Pharmacy, Integral University, Lucknow, India
  • Arshad Hussain College of Pharmacy, King Khalid University, Abha, KSA. Formerly, Faculty of Pharmacy, Integral University, Lucknow, India
  • - Badaruddeen Herbal Bioactive Research Laboratory, Faculty of Pharmacy, Integral University, Lucknow, India
  • Md Arshad Molecular Endocrinology Laboratory, Department of Zoology, University of Lucknow, Lucknow, India
  • Anuradha Mishra Herbal Bioactive Research Laboratory, Faculty of Pharmacy, Integral University, Lucknow, India

DOI:

https://doi.org/10.17179/excli2016-668

Keywords:

rutin, nanoemulsion, water titration method, cell viability, prostate cancer, reactive oxygen species

Abstract

Biological effects of rutin bioactive are limited due to its poor oral bioavailability and its degradation in aqueous environments. For the purpose of bioenhancement, different nanoemulsion systems of rutin were developed by aqueous titration method using water as dispersion media. The nanoemulsion systems were characterized for surface morphology, droplet size, polydispersity index, zeta potential, in vitro release profile and the formulations were optimized. The anticancer potential of optimized nanoemulsion was evaluated by cells viability (MTT) assay, nuclear condensation, and ROS activity using human prostate cancer (PC3) cell line. On the basis of cell viability data the inhibitory concentration (IC50) value for optimized nanoemulsion formulation on PC3 cancer cells was found to be 11.8 μM. Fluorescent microscopic analysis and intracellular ROS generation demonstrated significant ROS induction that might lead to triggering the apoptosis pathway. In conclusion, developed nanoemulsion displayed significant efficacy against prostate carcinoma cells.

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Published

2017-05-31

How to Cite

Ahmad, M., Sahabjada, .-., Akhtar, J., Hussain, A., Badaruddeen, .-., Arshad, M., & Mishra, A. (2017). Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line. EXCLI Journal, 16, 810–823. https://doi.org/10.17179/excli2016-668

Issue

Vol. 16 (2017)

Section

Original articles

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