Octyl gallate induces hepatic steatosis in HepG2 cells through the regulation of SREBP-1c and PPAR-γ gene expression

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Kelly Goulart Lima
Vitor Giancarlo Schneider Levorse
Maria Claudia Rosa Garcia
Bruno de Souza Basso
Bruna Pasqualotto Costa
Géssica Luana Antunes
Carolina Luft
Gabriela Viegas Haute
Léder Leal Xavier
Márcio Vinícius Fagundes Donadio
Jarbas Rodrigues de Oliveira

Abstract

Octyl gallate (OG) is an antioxidant commonly used in food, although there is no definition of its acceptable daily intake. There are reports in vitro and in vivo showing that food additives and drugs can alter lipid metabolism. Lipid droplet accumulation in hepatic cells is one of the main findings in the unregulated lipid metabolism and is strongly related to the development of nonalcoholic fatty liver disease (NAFLD). In this study, we investigated the effects of OG on lipid metabolism in the hepatocellular carcinoma cell line (HepG2). The results have shown, for the first time, that treatment with OG increased the overall amount of lipids, the triglyceride concentration, the lipid droplet area, and SREBP-1c and PPAR-γ gene expression. Taken together, the findings indicate that OG induces lipid droplet accumulation in HepG2 cells through the regulation of SREBP-1c and PPAR-γ gene expression without involving mTOR/S6K1 and may contribute to NAFLD when used as a food additive.

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How to Cite
Lima, K. G., Schneider Levorse, V. G., Rosa Garcia, M. C., de Souza Basso, B., Pasqualotto Costa, B., Antunes, G. L., Luft, C., Viegas Haute, G., Leal Xavier, L., Fagundes Donadio, M. V., & Rodrigues de Oliveira, J. (2020). Octyl gallate induces hepatic steatosis in HepG2 cells through the regulation of SREBP-1c and PPAR-γ gene expression. EXCLI Journal, 19, 962-971. https://doi.org/10.17179/excli2020-2214
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Original articles

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