MicroRNA expression analysis in endometriotic serum treated mesenchymal stem cells

Authors

  • Mazen Abdel-Rasheed Department of Reproductive Health Research, National Research Centre, Cairo, Egypt; Stem Cell Research group, Medical Research Centre of Excellence, National Research Centre, Cairo, Egypt
  • Ghada Nour Eldeen Department of Molecular Genetics and Enzymology, National Research Centre, Cairo, Egypt; Stem Cell Research group, Medical Research Centre of Excellence, National Research Centre, Cairo, Egypt
  • Marwa Mahmoud Department of Medical Molecular Genetics, National Research Centre, Cairo, Egypt; Stem Cell Research group, Medical Research Centre of Excellence, National Research Centre, Cairo, Egypt
  • Mahmoud ElHefnawi Biomedical Informatics and Chemo-informatics group, Informatics and Systems Department, National Research Centre, Cairo, Egypt
  • Nourhan Abu-Shahba Department of Medical Molecular Genetics, National Research Centre, Cairo, Egypt; Stem Cell Research group, Medical Research Centre of Excellence, National Research Centre, Cairo, Egypt
  • Mohamed Reda Department of Reproductive Health Research, National Research Centre, Cairo, Egypt; Stem Cell Research group, Medical Research Centre of Excellence, National Research Centre, Cairo, Egypt
  • Khaled Elsetohy Department of Obstetrics and Gynecology, Faculty of Medicine, Cairo University, Cairo, Egypt
  • Michael Nabil Department of Molecular Genetics, CliniLab, Cairo, Egypt
  • Amr Elnoury Department of Medical Applications of Laser, National Institute of Laser Enhanced Sciences, Cairo University, Cairo, Egypt
  • Tamer Taha Department of Reproductive Health Research, National Research Centre, Cairo, Egypt
  • Osama Azmy Department of Reproductive Health Research, National Research Centre, Cairo, Egypt; Stem Cell Research group, Medical Research Centre of Excellence, National Research Centre, Cairo, Egypt

DOI:

https://doi.org/10.17179/excli2017-101

Keywords:

endometriosis, mesenchymal stem cells, miRNA expression, differentiation

Abstract

Endometriosis is defined by presence of endometrial-like-tissue outside the uterus. Recently, ectopic endometriotic lesions have been suggested to originate by abnormal differentiation of endometrial mesenchymal stem cells (eMSCs). MicroRNAs (miRNAs) play an important role in the pathophysiology of endometriosis. Through a PCR array approach, we aimed to assess the differential expression of microRNAs in human eMSC treated in culture with sera derived from women with severe endometriosis. Sera were collected from five patients with severe endometriosis and three control women and added individually in the culture medium to conduct experimental and control eMSC sets, respectively. Regular microscopic follow-up for cell morphology was performed. SYBR Green based real-time PCR array was used to assess the expression of 84 miRNAs. Bioinformatics analysis was done to predict the target genes of the significantly dysregulated miRNAs and their enriched biological processes and pathways. Thirty-two miRNAs were found significantly dysregulated in experimental cultures. Functional enrichment analysis revealed several endometriosis associated biological processes and pathways were enriched by target genes of these miRNAs. In conclusion, treatment of human eMSCs with sera of severe endometriosis cases affects the expression of certain miRNAs and their target genes. This may result in altering cell functions and consequently, endometriosis development.

Downloads

Published

2017-06-14

How to Cite

Abdel-Rasheed, M., Nour Eldeen, G., Mahmoud, M., ElHefnawi, M., Abu-Shahba, N., Reda, M., … Azmy, O. (2017). MicroRNA expression analysis in endometriotic serum treated mesenchymal stem cells. EXCLI Journal, 16, 852–867. https://doi.org/10.17179/excli2017-101

Issue

Vol. 16 (2017)

Section

Original articles

License

Authors who publish in this journal agree to the following terms:

  • The authors keep the copyright and grant the journal the right of first publication under the terms of the Creative Commons Attribution license, CC BY 4.0. This licencse permits unrestricted use, distribution and reproduction in any medium, provided that the original work is properly cited.
  • The use of general descriptive names, trade names, trademarks, and so forth in this publication, even if not specifically identified, does not imply that these names are not protected by the relevant laws and regulations.
  • Because the advice and information in this journal are believed to be true and accurate at the time of publication, neither the authors, the editors, nor the publisher accept any legal responsibility for any errors or omissions presented in the publication. The publisher makes no guarantee, express or implied, with respect to the material contained herein.
  • The authors can enter into additional contracts for the non-exclusive distribution of the journal's published version by citing the initial publication in this journal (e.g. publishing in an institutional repository or in a book).