MafB silencing in macrophages does not influence the initiation and growth of lung cancer induced by urethane

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Takako Nemoto
Yoko Shibata
Sumito Inoue
Akira Igarashi
Yoshikane Tokairin
Keiko Yamauchi
Tomomi Kimura
Masamichi Sato
Kento Sato
Hiroshi Nakano
Shuichi Abe
Michiko Nishiwaki
Maki Kobayashi
Sujeong Yang
Yukihiro Minegishi
Kodai Furuyama
Hiroyoshi Machida
Isao Kubota

Abstract

An increased number of tumor-associated macrophages (TAMs) that exhibit the M2 macrophage phenotype is related to poorer prognosis in cancer patients. MafB is a transcription factor regulating the differentiation of macrophages. However, involvement of MafB for the development of TAMs is unknown. This study was designed to investigate the role of MafB in a murine urethane-induced lung cancer model. Urethane was injected intraperitoneally into wild-type and dominant-negative MafB transgenic mice. Twenty-four weeks later, mice were sacrificed and their lungs removed for pathological analysis. The numbers and mean areas of lung cancer were evaluated. In addition, the numbers of Mac-3-positive macrophages were evaluated in each tumor. The numbers and mean areas of lung cancer induced by urethane administration were not significantly different between wild-type and dominant-negative MafB transgenic mice. The numbers of TAMs in lung cancer tissue were not significantly different between the two groups. MafB silencing using dominant-negative MafB did not influence the initiation and growth of lung cancer in mice exposed to urethane. These data suggest that MafB may not be related to the development of TAMs.

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How to Cite
Nemoto, T., Shibata, Y., Inoue, S., Igarashi, A., Tokairin, Y., Yamauchi, K., Kimura, T., Sato, M., Sato, K., Nakano, H., Abe, S., Nishiwaki, M., Kobayashi, M., Yang, S., Minegishi, Y., Furuyama, K., Machida, H., & Kubota, I. (2017). MafB silencing in macrophages does not influence the initiation and growth of lung cancer induced by urethane. EXCLI Journal, 16, 914-920. https://doi.org/10.17179/excli2017-325
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