Prosopis juliflora leave extracts induce cell death of MCF-7, HepG2, and LS-174T cancer cell lines

Main Article Content

Serag Eldin I. Elbehairi
Ahmed Ezzat Ahmed
Ali A. Alshati
Mohammed A. Al-Kahtani
Mohammad Y. Alfaifi
Khalid M. Alsyaad
Ali Yahya A. Alalmie
Mohammed M. Elimam Ahamed
Mahmoud F. Moustafa
Sadeq K. Alhag
Ahmed M. Al-Abd
Ahmed M. Abbas


Prosopis juliflora (P. juliflora) is a widespread phreatophytic tree, which belongs to the Fabaceae family. The goal of the present study is to investigate the potential anti-cancer effect of P. juliflora leave extracts and to identify its chemical composition. For this purpose, MCF-7 (breast), HepG2 (liver), and LS-174T (colorectal) cancer cell lines were cultivated and incubated with various concentrations of P. juliflora leave extracts, and its impact on cell viability, proliferation, and cell cycle stages was investigated. P. juliflora leave extracts induced concentration-dependent cytotoxicity against all tested cancer cell lines. The calculated IC50 was 18.17, 33.1 and 41.9 μg/ml for MCF-7, HePG2 and LS-174T, respectively. Detailed analysis revealed that the cytotoxic action of P. juliflora extracts was mainly via necrosis but not apoptosis. Moreover, DNA content flow cytometry analysis showed cell-specific anti-proliferative action and cell cycle stages arrest. In order to identify the anti-cancer constituents of P. juliflora, the ethyl extracts were analyzed by liquid chromatography-mass spectrometry. The major constituents identified in the ethyl extracts of P. juliflora leaves were hydroxymethyl-pyridine, nicotinamide, adenine, and poly-(methyl methacrylate) (PMMA). In conclusion, P. juliflora ethyl acetate extracts have a potential anti-cancer effect against breast adenocarcinoma, hepatocellular carcinoma, and colorectal adenocarcinoma, and is enriched with anti-cancer constituents.

Article Details

How to Cite
Elbehairi, S. E. I., Ezzat Ahmed, A., Alshati, A. A., Al-Kahtani, M. A., Alfaifi, M. Y., Alsyaad, K. M., Alalmie, A. Y. A. ., Elimam Ahamed, M. M., Moustafa, M. F., Alhag, S. K., Al-Abd, A. M., & Abbas, A. M. (2020). Prosopis juliflora leave extracts induce cell death of MCF-7, HepG2, and LS-174T cancer cell lines. EXCLI Journal, 19, 1282-1294.
Original articles