Prosopis juliflora leave extracts induce cell death of MCF-7, HepG2, and LS-174T cancer cell lines

Authors

  • Serag Eldin I. Elbehairi Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia; Cell Culture Laboratory, Egyptian Organization for Biological Products and Vaccines (VACSERA Holding Company), Agouza, Giza, Egypt
  • Ahmed Ezzat Ahmed Department of Biology, College of Science, King Khalid University, 61413 Abha, Saudi Arabia, Tel.: +966 0583414564; E-mail: aabdelrahman@kku.edu.sa; ahmed.ezzat@vet.svu.edu.eg; Department of Theriogenology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt
  • Ali A. Alshati Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia
  • Mohammed A. Al-Kahtani Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia
  • Mohammad Y. Alfaifi Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia
  • Khalid M. Alsyaad Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia; Director of the Research Centre, College of Science, King Khalid University, Abha, Saudi Arabia
  • Ali Yahya A. Alalmie The Poison Control and Medical Forensic Chemistry Centre, Asir, Saudi Arabia
  • Mohammed M. Elimam Ahamed Department of Chemistry, College of Science, King Khalid University, Abha, Saudi Arabia; Unit of Bee Research and Honey Production, College of Science, King Khalid University, Abha, Saudi Arabia
  • Mahmoud F. Moustafa Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia; Department of Botany & Microbiology, Faculty of Science, South Valley University, Qena, Egypt
  • Sadeq K. Alhag Biology Department, College of Arts and Science, King Khalid University, Muhayl Asir, Saudi Arabia; Biology Department, College of Science, Ibb University, Yemen
  • Ahmed M. Al-Abd Department of Pharmaceutical Science, College of Pharmacy & Thumbay Research Institute of Precision Medicine, Gulf Medical University, Ajman, UAE; Pharmacology Department, Medical Division, National Research Centre, Cairo, Egypt
  • Ahmed M. Abbas Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia; Department of Botany & Microbiology, Faculty of Science, South Valley University, Qena, Egypt

DOI:

https://doi.org/10.17179/excli2020-2830

Keywords:

Prosopis juliflora, cytotoxicity, apoptosis, cancer, MCF-7, HepG2, LS-174T

Abstract

Prosopis juliflora (P. juliflora) is a widespread phreatophytic tree, which belongs to the Fabaceae family. The goal of the present study is to investigate the potential anti-cancer effect of P. juliflora leave extracts and to identify its chemical composition. For this purpose, MCF-7 (breast), HepG2 (liver), and LS-174T (colorectal) cancer cell lines were cultivated and incubated with various concentrations of P. juliflora leave extracts, and its impact on cell viability, proliferation, and cell cycle stages was investigated. P. juliflora leave extracts induced concentration-dependent cytotoxicity against all tested cancer cell lines. The calculated IC50 was 18.17, 33.1 and 41.9 μg/ml for MCF-7, HePG2 and LS-174T, respectively. Detailed analysis revealed that the cytotoxic action of P. juliflora extracts was mainly via necrosis but not apoptosis. Moreover, DNA content flow cytometry analysis showed cell-specific anti-proliferative action and cell cycle stages arrest. In order to identify the anti-cancer constituents of P. juliflora, the ethyl extracts were analyzed by liquid chromatography-mass spectrometry. The major constituents identified in the ethyl extracts of P. juliflora leaves were hydroxymethyl-pyridine, nicotinamide, adenine, and poly-(methyl methacrylate) (PMMA). In conclusion, P. juliflora ethyl acetate extracts have a potential anti-cancer effect against breast adenocarcinoma, hepatocellular carcinoma, and colorectal adenocarcinoma, and is enriched with anti-cancer constituents.

Published

2020-09-09

How to Cite

Elbehairi, S. E. I., Ezzat Ahmed, A., Alshati, A. A., Al-Kahtani, M. A., Alfaifi, M. Y., Alsyaad, K. M., Alalmie, A. Y. A. ., Elimam Ahamed, M. M., Moustafa, M. F., Alhag, S. K., Al-Abd, A. M., & Abbas, A. M. (2020). Prosopis juliflora leave extracts induce cell death of MCF-7, HepG2, and LS-174T cancer cell lines. EXCLI Journal, 19, 1282–1294. https://doi.org/10.17179/excli2020-2830

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Section

Original articles