Separation of glycine-rich proteins from sea hare eggs and their anti-cancer activity against U937 leukemia cell line

Authors

  • Won Woo Lee School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Republic of Korea
  • Won-Suck Kim College of Medical and Life Sciences, Silla University, Busan, 46958, Republic of Korea
  • Ginnae Ahn Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 59626, Republic of Korea
  • Kil-Nam Kim Jeju center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea
  • Soo-Jin Heo Global Bioresources Research Center, Korea Institute of Ocean Science & Technology, Jeju, Republic of Korea
  • Moonjae Cho Department of Biochemistry, College of Medicine, Cheju National University, Jeju 63349, Republic of Korea
  • I. P. Shanura Fernando School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Republic of Korea
  • Nalae Kang School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Republic of Korea
  • You-Jin Jeon School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Republic of Korea

DOI:

https://doi.org/10.17179/excli2016-293

Keywords:

sea hare egg (SE), glycine-rich protein, anti-cancer effect, apoptosis, mitochondrial pathway

Abstract

The present study was designed to investigate the anti-cancer effects of Sea hare eggs (SE) in U937 cells and its major active components. The aqueous extract of SE (ASE), which contained the highest protein content, dose-dependently inhibited the cancer cell's growth (IC50 value, 10.42 ± 0.5 µg/mL). Additionally, ASE markedly caused DNA damage by inducing apoptotic body formation, DNA fragmentation, and accumulation of sub-G1 DNA contents. ASE induced apoptosis by activating caspase-3 and 9 and poly (ADP-ribose) polymerase (PARP) by regulating the expression of Bcl-2/Bax. Moreover, among its molecular weight fractions, the > 30 kDa fraction showed the highest cell-growth-inhibitory effects, which was inhibited by heat treatment. Furthermore, the > 30 kDa fraction had markedly higher glycine content than the ASE. The presence of two protein bands at around 16 and 32 kDa was identified. In addition, two fractions, F1 and F2, were obtained using anion-exchange chromatography, with the F1 having an improved cell-growth-inhibitory effect than the > 30 kDa fraction. Taken together, these results suggest that the ASE contains glycine-rich proteins, including the active 16 and 32 kDa proteins, which account for its anti-cancer effects by inducing apoptosis via regulation of the mitochondrial pathway.

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Published

2016-06-01

How to Cite

Lee, W. W., Kim, W.-S., Ahn, G., Kim, K.-N., Heo, S.-J., Cho, M., … Jeon, Y.-J. (2016). Separation of glycine-rich proteins from sea hare eggs and their anti-cancer activity against U937 leukemia cell line. EXCLI Journal, 15, 329–342. https://doi.org/10.17179/excli2016-293

Issue

Vol. 15 (2016)

Section

Original articles

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