Separation of glycine-rich proteins from sea hare eggs and their anti-cancer activity against U937 leukemia cell line

Main Article Content

Won Woo Lee
Won-Suck Kim
Ginnae Ahn
Kil-Nam Kim
Soo-Jin Heo
Moonjae Cho
I. P. Shanura Fernando
Nalae Kang
You-Jin Jeon

Abstract

The present study was designed to investigate the anti-cancer effects of Sea hare eggs (SE) in U937 cells and its major active components. The aqueous extract of SE (ASE), which contained the highest protein content, dose-dependently inhibited the cancer cell's growth (IC50 value, 10.42 ± 0.5 µg/mL). Additionally, ASE markedly caused DNA damage by inducing apoptotic body formation, DNA fragmentation, and accumulation of sub-G1 DNA contents. ASE induced apoptosis by activating caspase-3 and 9 and poly (ADP-ribose) polymerase (PARP) by regulating the expression of Bcl-2/Bax. Moreover, among its molecular weight fractions, the > 30 kDa fraction showed the highest cell-growth-inhibitory effects, which was inhibited by heat treatment. Furthermore, the > 30 kDa fraction had markedly higher glycine content than the ASE. The presence of two protein bands at around 16 and 32 kDa was identified. In addition, two fractions, F1 and F2, were obtained using anion-exchange chromatography, with the F1 having an improved cell-growth-inhibitory effect than the > 30 kDa fraction. Taken together, these results suggest that the ASE contains glycine-rich proteins, including the active 16 and 32 kDa proteins, which account for its anti-cancer effects by inducing apoptosis via regulation of the mitochondrial pathway.

Article Details

How to Cite
Lee, W. W., Kim, W.-S., Ahn, G., Kim, K.-N., Heo, S.-J., Cho, M., Fernando, I. P. S., Kang, N., & Jeon, Y.-J. (2016). Separation of glycine-rich proteins from sea hare eggs and their anti-cancer activity against U937 leukemia cell line. EXCLI Journal, 15, 329-342. https://doi.org/10.17179/excli2016-293
Section
Original articles

Most read articles by the same author(s)