Anti-inflammatory effect of supercritical extract and its constituents from Ishige okamurae

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Eun-Yi Ko
Weon-Jong Yoon
Hae-Won Lee
Soo-Jin Heo
Young-Hwan Ko
I.P. Shanura Fernando
Kichul Cho
Chi-Heon Lee
Sung-Pyo Hur
Su-Hyeon Cho
Ginnae Ahn
Daekyung Kim
Kil-Nam Kim

Abstract

The anti-inflammatory properties of the supercritical fluid extract of Ishige okamurae (SFEIO) on lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. The lipid profile of the SFEIO, reviled the presence of palmitic acid (220.2 mg/g), linoleic acid (168.0 mg/g), and oleic acid (123.0 mg/g). SFEIO was found to exert it's anti-inflammatory effects through inhibiting nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 production in LPS-stimulated RAW 264.7 cells, without inducing cytotoxicity. SFEIO did not effect on the LPS-induced p38 kinase phosphorylation, whereas it attenuated the extracellular-related signaling kinase (ERK) and c-Jun N-terminal kinase (JNK) phosphorylation. Furthermore, SFEIO inhibited the LPS-induced IκB-α degradation and p50 NF-κB activation. These results suggest that SFEIO exerts its anti-inflammatory effects in LPS-activated RAW 264.7 cells by down-regulating the activation of ERK, JNK, and NF-κB.

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How to Cite
Ko, E.-Y., Yoon, W.-J., Lee, H.-W., Heo, S.-J., Ko, Y.-H., Fernando, I. S., Cho, K., Lee, C.-H., Hur, S.-P., Cho, S.-H., Ahn, G., Kim, D., & Kim, K.-N. (2016). Anti-inflammatory effect of supercritical extract and its constituents from Ishige okamurae. EXCLI Journal, 15, 434-445. https://doi.org/10.17179/excli2016-337
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Original articles

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