Phlomis fruticosa L. exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line

Authors

  • Dejan Stojković Department of Plant Physiology, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11000 Belgrade, Serbia https://orcid.org/0000-0002-4159-1471
  • Danijela Drakulić Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11042 Belgrade 152, Serbia https://orcid.org/0000-0001-6790-6673
  • Maria Inês Dias Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal
  • Gokhan Zengin Physiology and Biochemistry Research Laboratory, Department of Biology, Science Faculty, Selcuk University, Campus, 42130, Konya, Turkey https://orcid.org/0000-0001-6548-7823
  • Lillian Barros Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal https://orcid.org/0000-0002-9050-5189
  • Marija Ivanov Department of Plant Physiology, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11000 Belgrade, Serbia https://orcid.org/0000-0002-2480-5490
  • Uroš Gašić Department of Plant Physiology, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11000 Belgrade, Serbia https://orcid.org/0000-0001-5384-8396
  • Nemanja Rajčević University of Belgrade, Faculty of Biology, Studentski trg 16, 11158 Belgrade, Serbia https://orcid.org/0000-0003-2260-1205
  • Milena Stevanović Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11042 Belgrade 152, Serbia; University of Belgrade, Faculty of Biology, Studentski trg 16, 11158 Belgrade, Serbia; Serbian Academy of Sciences and Arts, Kneza Mihaila 35, 11000 Belgrade, Serbia https://orcid.org/0000-0003-4286-7334
  • Isabel C. F. R. Ferreira Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal https://orcid.org/0000-0003-4910-4882
  • Marina Soković Department of Plant Physiology, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11000 Belgrade, Serbia; Tel: +381-11-207-84-19; Fax: +381-11-2-761-433; E-mail: mris@ibiss.bg.ac.rs https://orcid.org/0000-0002-7381-756X

DOI:

https://doi.org/10.17179/excli2021-4487

Keywords:

Phlomis fruticosa, antioxidant, antineurodegenerative, glioblastoma, prooxidant, phenolic compounds

Abstract

Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehensively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenerative disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa extract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic effect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 μg/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.

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Published

2022-02-14

How to Cite

Stojković, D., Drakulić, D., Dias, M. I., Zengin, G., Barros, L., Ivanov, M., … Soković, M. (2022). Phlomis fruticosa L. exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line. EXCLI Journal, 21, 387–399. https://doi.org/10.17179/excli2021-4487

Issue

Vol. 21 (2022)

Section

Original articles

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Copyright (c) 2022 Dejan Stojković, Danijela Drakulić, Maria Inês Dias, Gokhan Zengin, Lillian Barros, Marija Ivanov, Uroš Gašić, Nemanja Rajčević, Milena Stevanović, Isabel C. F. R. Ferreira, Marina Soković

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