Low-toxic herbicides Roundup and Atrazine disturb free radical processes in Daphnia in environmentally relevant concentrations

Authors

  • Viktor Husak Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenka Str., Ivano-Frankivsk, 76018, Ukraine https://orcid.org/0000-0001-9415-9837
  • Tetiana Strutynska Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenka Str., Ivano-Frankivsk, 76018, Ukraine https://orcid.org/0000-0003-2854-1303
  • Nadia Burdyliuk Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenka Str., Ivano-Frankivsk, 76018, Ukraine https://orcid.org/0000-0001-9590-3672
  • Anzhelika Pitukh Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenka Str., Ivano-Frankivsk, 76018, Ukraine https://orcid.org/0000-0002-7556-3049
  • Volodymyr Bubalo Laboratory of Experimental Toxicology and Mutagenesis, L.I. Medved’s Research Center of Preventive Toxicology, Food and Chemical Safety, MHU, Kyiv, Ukraine https://orcid.org/0000-0003-0230-9354
  • Halyna Falsfushynska Ternopil Volodymyr Hnatiuk National Pedagogical University, M. Kryvonosa, 2, Ternopil, 46027, Ukraine https://orcid.org/0000-0002-6299-1853
  • Olha Strilbytska Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenka Str., Ivano-Frankivsk, 76018, Ukraine https://orcid.org/0000-0003-3277-2294
  • Oleh Lushchak Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine. Tel/Fax.: +380342714683; E-mail: oleh.lushchak@pnu.edu.ua https://orcid.org/0000-0002-4627-1987

DOI:

https://doi.org/10.17179/excli2022-4690

Keywords:

Roundup, Atrazine, Daphnia, oxidative stress, toxicity

Abstract

The use of glyphosate-based Roundup and triazine herbicide Atrazine has increased markedly in last decades. Thus, it is important to evaluate toxic effects of these herbicides to non-targeted organisms such as zooplankton to understand their safety toward aquatic ecosystems. In the current study, we performed Daphnia toxicity tests based on lethality to identify LC50 that provides acute aquatic toxicity classification criteria. LC50 for Roundup exposure for 24 hours was found to be 0.022 mg/L and 48 hours - 0.0008 mg/L. Atrazine showed LC50 at concentrations of 40 mg/L and 7 mg/L for 24 and 48 hours, respectively. We demonstrated that exposure to ecologically relevant concentrations of Roundup or Atrazine decreases lipid peroxidation and protein thiol levels, however caused increase in carbonyl protein and low-molecular-weight thiols content. Moreover, the herbicide treatments caused increase of superoxide dismutase activity. Our data suggest that at very low concentrations Roundup and Atrazine disturb free radical processes in D. magna.

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Published

2022-03-09

How to Cite

Husak, V., Strutynska, T., Burdyliuk, N., Pitukh, A., Bubalo, V., Falsfushynska, H., … Lushchak, O. (2022). Low-toxic herbicides Roundup and Atrazine disturb free radical processes in Daphnia in environmentally relevant concentrations. EXCLI Journal, 21, 595–609. https://doi.org/10.17179/excli2022-4690

Issue

Vol. 21 (2022)

Section

Original articles

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Copyright (c) 2022 Viktor Husak, Tetiana Strutynska, Nadia Burdyliuk, Anzhelika Pitukh, Volodymyr Bubalo, Halyna Falsfushynska, Olha Strilbytska, Oleh Lushchak

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