Transient receptor potential channel involvement in antinociceptive effect of citral in orofacial acute and chronic pain models

Authors

  • Sacha Aubrey Alves Rodrigues Santos Universidade de Fortaleza, Núcleo de Biologia Experimental, Av. Washington Soares, 1321, Edson Queiroz – Fortaleza, Ceará, Brasil, Tel. +55 85 3477 3846; E-mail: sachaaubrey@hotmail.com https://orcid.org/0000-0002-4117-2277
  • Marina de Barros Mamede Vidal Damasceno Experimental Biology Center, University of Fortaleza, Fortaleza, Brazil https://orcid.org/0000-0002-0276-2549
  • Francisco Ernani Alves Magalhães Experimental Biology Center, University of Fortaleza, Fortaleza, Brazil; Department of Nutrition and Health, State University of Ceará, Fortaleza, Brazil https://orcid.org/0000-0002-4924-2882
  • Barry John Sessle Department of Physiology and Faculty of Dentistry, University of Toronto, Toronto, Canada https://orcid.org/0000-0001-5334-7460
  • Breytiner Amaro de Oliveira Experimental Biology Center, University of Fortaleza, Fortaleza, Brazil https://orcid.org/0000-0001-6844-4677
  • Francisco Lucas Alves Batista Experimental Biology Center, University of Fortaleza, Fortaleza, Brazil https://orcid.org/0000-0002-7543-057X
  • Antônio Eufrásio Vieira-Neto Experimental Biology Center, University of Fortaleza, Fortaleza, Brazil https://orcid.org/0000-0001-7003-6461
  • Adriana Rolim Campos Experimental Biology Center, University of Fortaleza, Fortaleza, Brazil https://orcid.org/0000-0002-7355-9310

DOI:

https://doi.org/10.17179/excli2022-5042

Keywords:

Citral, TRP channels, orofacial nociception

Abstract

This study aimed to test for the possible antinociceptive effect of the naturally occurring terpene citral in rodent models of acute and chronic orofacial pain and to test for the possible involvement of transient receptor potential (TRP) channels in this effect. Acute nociceptive behavior was induced in one series of experiments by administering formalin, cinnamaldehyde, menthol or capsaicin to the upper lip. Nociceptive behavior was assessed by orofacial rubbing, and the effects of pre-treatment with citral (0.1, 0.3 or 1.0 mg/Kg) or vehicle (control) were tested on the behavior. Nociceptive behavior was also induced by formalin injected into the temporomandibular joint or mustard oil injected into the masseter muscle, preceded by citral or vehicle (control) treatment. The chronic pain model involved infraorbital nerve transection (IONX) that induced mechanical hypersensitivity which was assessed by von Frey hair stimulation of the upper lip. Motor activity was also evaluated. Docking experiments were performed using TRPV1 and TRPM8 channels. Citral but not vehicle produced significant (p<0.01, ANOVA) antinociception on all the acute nociceptive behaviors, and these effects were attenuated by TRPV1 antagonist capsazepine, TRPM3 antagonist mefenamic acid and by TRPM8 desensitization, but not by ruthenium red and TRPA1 antagonist HC-030031. The IONX animals developed facial mechanical hypersensitivity that was significantly reduced by citral but not by vehicle. The docking experiments revealed that citral may interact with TRPV1 and TRPM8 channels. These results indicate the potential use of citral as an inhibitor of orofacial nociception in both acute and chronic pain states through TRPV1, TRPM3 and TRPM8 channels.

Downloads

Published

2022-06-24

How to Cite

Alves Rodrigues Santos, S. A., de Barros Mamede Vidal Damasceno, M., Alves Magalhães, F. E., Sessle, B. J., Amaro de Oliveira, B., Alves Batista, F. L., Vieira-Neto, A. E., & Rolim Campos, A. (2022). Transient receptor potential channel involvement in antinociceptive effect of citral in orofacial acute and chronic pain models. EXCLI Journal, 21, 869–887. https://doi.org/10.17179/excli2022-5042

Issue

Vol. 21 (2022)

Section

Original articles

Categories

License

Copyright (c) 2022 Sacha Aubrey Alves Rodrigues Santos, Marina de Barros Mamede Vidal Damasceno, Francisco Ernani Alves Magalhães, Barry John Sessle, Breytiner Amaro de Oliveira, Francisco Lucas Alves Batista, Antônio Eufrásio Vieira-Neto, Adriana Rolim Campos

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish in this journal agree to the following terms:

  • The authors keep the copyright and grant the journal the right of first publication under the terms of the Creative Commons Attribution license, CC BY 4.0. This licencse permits unrestricted use, distribution and reproduction in any medium, provided that the original work is properly cited.
  • The use of general descriptive names, trade names, trademarks, and so forth in this publication, even if not specifically identified, does not imply that these names are not protected by the relevant laws and regulations.
  • Because the advice and information in this journal are believed to be true and accurate at the time of publication, neither the authors, the editors, nor the publisher accept any legal responsibility for any errors or omissions presented in the publication. The publisher makes no guarantee, express or implied, with respect to the material contained herein.
  • The authors can enter into additional contracts for the non-exclusive distribution of the journal's published version by citing the initial publication in this journal (e.g. publishing in an institutional repository or in a book).