Correlation of miR-24-3p and miR-595 expression with CCL3, CCL4, IL1-β, TNFαIP3, and NF-κBIα genes in PBMCs of patients with coronary artery disease

Authors

  • Shakiba Kazemian Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran https://orcid.org/0000-0003-4689-3628
  • Reza Ahmadi Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran https://orcid.org/0000-0003-1623-0559
  • Gordon A. Ferns Brighton & Sussex Medical School, Division of Medical Education, Falmer, Brighton, Sussex, UK https://orcid.org/0000-0002-0957-8349
  • Ali Rafiei Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran https://orcid.org/0000-0001-5180-7662
  • Fatemeh Azadegan-Dehkordi Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran https://orcid.org/0000-0003-4469-8028
  • Arsalan Khaledifar Department of Cardiology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran https://orcid.org/0000-0003-2323-171X
  • Mina Mohammad-Rezaei Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran https://orcid.org/0000-0001-6977-3528
  • Nader Bagheri Department of Microbiology and Immunology, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran. Tel: +989181731073; Fax: +98-3813330709. E-mail: n.bagheri1985@gmail.com https://orcid.org/0000-0001-5196-5513

DOI:

https://doi.org/10.17179/excli2022-5266

Keywords:

atherosclerosis, cardiovascular diseases, inflammation, miR-24-3p, miR-595, NF-κBIα

Abstract

Inflammation has been well recognized to play an important role in developing coronary artery disease (CAD). By regulating essential genes in this pathway post-transcriptionally, MicroRNAs (miRNAs) may help or hinder the development of atherosclerotic lesions. The aim of this study was to investigate the expression of miR-24-3p, miR-595, CCL3, CCL4, IL-1β, TNFαIP3, and NF-κBIα in the peripheral blood mononuclear cells (PBMCs) of CAD and control groups and to examine whether any correlation exists between the expression of miRs and genes in CAD group. A total of 168 subjects (84 CAD subjects and 84 control subjects) were examined in this research. Expression levels of miR-24-3p, miR-595, CCL3, CCL4, IL-1β, TNFαIP3, and NF-κBIα in PBMCs were measured using the real-time PCR technique. A comparison of the CAD group with the control group indicated significantly increased expression levels of CCL3, CCL4, and IL-1β (Fold Change (FC)=4, P=0.009; FC=2.9, P=0.01; FC=1.8, P=0.019, respectively) and remarkably reduced expression levels of TNFαIP3 and NF-κBIα (FC=-1.4, P=0.03 and FC=-5.9, P=0.001, respectively). Moreover, the expression levels of miR-24-3p downregulated (FC=-2.5, P=0.005) and miR-595 upregulated (FC=1.9, P=0.009) in the CAD group. There was a statistical correlation between the number of clogged arteries with expression levels of miR-24-3p, miR-595, CCL3, CCL4, IL-1β, TNFαIP3, and NF-κBIα in the CAD group. Also, there was a statistical correlation between expression levels of miR-24-3p and miR-595 with CCL3, CCL4, IL-1β, TNFαIP3, and NF-κBIα gene expression in the CAD group. In CAD patients, decreased expression of miR-24-3p and increased expression of miR-595 may aid the progression of atherosclerotic plaques by regulating CCL3, CCL4, IL-1β, TNFαIP3, and NF-κBIα gene expression.

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Published

2022-09-08

How to Cite

Kazemian, S., Ahmadi, R., Ferns, G. A., Rafiei, A., Azadegan-Dehkordi, F., Khaledifar, A., Mohammad-Rezaei, M., & Bagheri, N. (2022). Correlation of miR-24-3p and miR-595 expression with CCL3, CCL4, IL1-β, TNFαIP3, and NF-κBIα genes in PBMCs of patients with coronary artery disease. EXCLI Journal, 21, 1184–1195. https://doi.org/10.17179/excli2022-5266

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