Antihypercholesterolemic effect of Cleome arabica L. on high cholesterol diet induced damage in rats

Authors

  • Noura Samout Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5- PB n° 1171-3000, Sfax, Tunisia; Laboratory Animal Eco Physiology, Faculty of Sciences of Gafsa, Sidi Ahmed Zarrouk, 2112, Gafsa, Tunisia
  • Hafsia Bouzenna Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5- PB n° 1171-3000, Sfax, Tunisia; Laboratory Animal Eco Physiology, Faculty of Sciences of Gafsa, Sidi Ahmed Zarrouk, 2112, Gafsa, Tunisia
  • Amani Ettaya Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5- PB n° 1171-3000, Sfax, Tunisia; Laboratory Animal Eco Physiology, Faculty of Sciences of Gafsa, Sidi Ahmed Zarrouk, 2112, Gafsa, Tunisia
  • Abdelfattah Elfeki Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5- PB n° 1171-3000, Sfax, Tunisia
  • Najla Hfaiedh Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5- PB n° 1171-3000, Sfax, Tunisia; Laboratory Animal Eco Physiology, Faculty of Sciences of Gafsa, Sidi Ahmed Zarrouk, 2112, Gafsa, Tunisia

DOI:

https://doi.org/10.17179/excli2015-169

Keywords:

hypercholesterolemic rat, Cleome arabica, lipid peroxidation (TBARS), antioxidant enzymes

Abstract

Dietary cholesterol is known to be one of the main risk factors that accelerate oxidation process leading to hypercholesterolemia and attendant cardiovascular diseases. The purpose of this study, carried out on adult male Wistar rats, was to evaluate the inhibitory effects of supplementation with aqueous of Cleome arabica leaf extract on hypercholesterolemia. After 3 months of treatment, animals were sacrificed by decapitation. Blood serum was obtained by centrifugation. Under our experimental conditions, administration of Cleome arabica leaf extract decreased the total cholesterol (TC), LDL-cholesterol (LDL-chol) and triglycerides (TG) levels by 27 %, 52 %, 37 %, respectively, and reduced SGOT SGPT, LDH and PAL levels in blood serum compared to untreated hypercholesterolemic rats. TBARS concentrations decreased by 21 % in liver, 22 % in heart and 30 % in kidney in a group of rats treated with cholesterol and Cleome arabica (Chol C.ar) compared to a Chol-treated group. The same treatment with Cleome arabica leaf extract increased superoxide dismutase and enhanced glutathione peroxidase activity. Catalase activity was found to increase in liver, heart and kidney by 17 %, 16 % and 23 %, respectively, in the C.ar Chol-treated group. The protective effect of Cleome arabica on hypercholesterolemia inducing oxidative stress in several organs was mainly attributed to antioxidant properties. The latter were due to the presence of phenolic acids and flavonoids shown by the obtained HPLC profiles.

Published

2015-07-06

How to Cite

Samout, N., Bouzenna, H., Ettaya, A., Elfeki, A., & Hfaiedh, N. (2015). Antihypercholesterolemic effect of Cleome arabica L. on high cholesterol diet induced damage in rats. EXCLI Journal, 14, 791–800. https://doi.org/10.17179/excli2015-169

Issue

Section

Original articles