Trans-1,3-diphenyl-2,3-epoxypropan-1-one, a chalcone derivative, induces apoptosis via ROS-mediated down-regulation of Bcl-xL in human leukemia HL-60 cells

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Eun-Yi Ko
Seung-Hong Lee
Ji-Yeon Ko
Jeong Yong Moon
Weon-Jong Yoon
Ginnae Ahn
Seong Woon Roh
Kichul Cho
You-Jin Jeon
Daekyung Kim
Kil-Nam Kim

Abstract

The anticancer effects of trans-1,3-diphenyl-2,3-epoxypropan-1-one (DPEP), a chalcone derivative, were investigated in human leukemia HL-60 cells. Treatment of HL-60 cells with various concentration of DPEP resulted in a sequence of events characteristic of apoptosis, including loss of cell viability, morphological changes, and increased sub-G1 DNA content. We demonstrated that DPEP elevates reactive oxygen species (ROS) levels in HL-60 cells, and that the ROS scavenger N-acetylcysteine (NAC) could block DPEP-induced ROS generation and apoptosis. Western blot analysis revealed that DPEP inhibits Bcl-xL expression, leading to caspase-3 activation and poly-ADP-ribose polymerase (PARP) cleavage, thereby inducing apoptosis. However, NAC pre-treatment significantly inhibited the activation of caspase-3 and PARP cleavage and reduced Bcl-xL levels. These findings provide the first evidence that DPEP may inhibit the growth of HL-60 cells and induce apoptosis through a ROS-mediated Bcl-xL pathway.

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How to Cite
Ko, E.-Y., Lee, S.-H., Ko, J.-Y., Moon, J. Y., Yoon, W.-J., Ahn, G., Roh, S. W., Cho, K., Jeon, Y.-J., Kim, D., & Kim, K.-N. (2015). Trans-1,3-diphenyl-2,3-epoxypropan-1-one, a chalcone derivative, induces apoptosis via ROS-mediated down-regulation of Bcl-xL in human leukemia HL-60 cells. EXCLI Journal, 14, 900-907. https://doi.org/10.17179/excli2015-373
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Original articles

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