Biochemical and immunological parameters as indicators of osteoarthritis subjects: role of OH-collagen in auto-antibodies generation

Authors

  • Jalaluddin M. Ashraf School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea
  • Quazi S. Haque Department of Biochemistry, Hind Institute of Medical Sciences, Barabanki, U.P., India
  • Shams Tabrez King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia
  • Inho Choi School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea
  • Saheem Ahmad Department of BioSciences, Integral University, Lucknow, India

DOI:

https://doi.org/10.17179/excli2014-423

Keywords:

osteoarthritis, collagen-II, biochemical and immunological parameters

Abstract

Osteoarthritis (OA) is characterized by inflammation of the knee joint, which is caused by accumulation of cytokines and C-reactive protein (CRP) in the extracellular matrix as an early immune response to infection. The articular cartilage destruction is discernible by elevated tumour necrosis factor-α (TNF-α). In this study, blood samples of knee osteoarthritis patients were analyzed for biochemical and physiological parameters based on the lipid profile, uric acid, total leukocyte count (TLC), hemoglobin percentage (Hb%) and absolute lymphocyte count (ALC). Furthermore, immunological parameters including TNF-α , interleukin-6 (IL-6) and CRP were analyzed. The presence of antibodies against hydroxyl radical modified collagen-II (OH-collagen-II) was also investigated in arthritis patients using direct binding ELISA. The uric acid and lipid profiles changed extensively. Specifically, increased uric acid levels were associated with OA in both genders, as were enhanced immunological parameters. The TNF-α level also increased in both genders suffering from OA. Finally, auto-antibodies against OH-collagen II antigen were found in the sera of arthritis patients. These results indicated that immunological parameters are better predictors or indexes for diagnosis of OA than biochemical parameters.

Published

2015-09-23

How to Cite

Ashraf, J. M., Haque, Q. S., Tabrez, S., Choi, I., & Ahmad, S. (2015). Biochemical and immunological parameters as indicators of osteoarthritis subjects: role of OH-collagen in auto-antibodies generation. EXCLI Journal, 14, 1057–1066. https://doi.org/10.17179/excli2014-423

Issue

Section

Original articles

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