Thymoquinone and curcumin attenuate gentamicin-induced renal oxidative stress, inflammation and apoptosis in rats

Authors

  • Ayman M Mahmoud Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Egypt
  • Osama M Ahmed Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Egypt
  • Sanaa R Galaly Cell Biology and Histology Division, Zoology Department, Faculty of Science, Beni-Suef University, Egypt

Keywords:

gentamicin, nephrotoxicity, thymoquinone, curcumin, inflammation, apoptosis, Bcl-2

Abstract

The present study was aimed to investigate the possible protective effects of thymoquinone (TQ) and curcumin (Cur) on gentamicin (GM)-induced nephrotoxicity in rats. Rats were divided into four groups as follows: group 1 received normal saline and served as normal controls, group 2 received GM only, group 3 concurrently received GM and TQ and group 4 concurrently received GM and Cur. At day 21, rats were sacrificed and samples were collected for assaying serum tumor necrosis factor alpha (TNF-α), urea and creatinine levels, and renal lipid peroxidaion, glutathione (GSH) content as well as glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities. In addition, kidneys were collected for histopathological examination and immunohistochemical determination of the antiapoptotic protein, B-cell lymphoma 2 (Bcl-2). The biochemical results showed that GM-induced nephrotoxicity was associated with a significant increase in serum TNF-α, urea and creatinine as well as renal lipid peroxidation. On the other hand, renal GSH content and GPx and SOD activities were significantly declined. Concomitant administration of either TQ or Cur efficiently alleviated the altered biochemical and histopathological features. In conclusion, both TQ and Cur showed more or less similar marked renoprotective effect against GM-induced nephrotoxicity through their antioxidant, anti-inflammatory and anti-apoptotic efficacies.

Published

2014-02-13

How to Cite

Mahmoud, A. M., Ahmed, O. M., & Galaly, S. R. (2014). Thymoquinone and curcumin attenuate gentamicin-induced renal oxidative stress, inflammation and apoptosis in rats. EXCLI Journal, 13, 98–110. Retrieved from https://www.excli.de/excli/article/view/685

Issue

Section

Original articles