Immunomodulatory potential of a bioactive fraction from the leaves of Phyllostachys bambusoides (bamboo) in BALB/c mice

Authors

  • Sunil Kumar Department of Pharmacology, School of Pharmaceutical Sciences Shoolini University Solan (H.P.) 173229, India
  • Gaurav Sharma Department of Pharmacology, School of Pharmaceutical Sciences Shoolini University Solan (H.P.) 173229, India
  • Tabasum Sidiq Division of Pharmacology, Indian Institute of Integrative Medicine (CSIR), Jammu 180001, India
  • Anamika Khajuria Division of Pharmacology, Indian Institute of Integrative Medicine (CSIR), Jammu 180001, India
  • Mahendra Jain Department of Pharmacognosy, School of Pharmaceutical Sciences Shoolini University Solan (H.P.) 173229, India
  • Deepak Bhagwat Department of Pharmacology, School of Pharmaceutical Sciences Shoolini University Solan (H.P.) 173229, India
  • KL Dhar Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences Shoolini University Solan (H.P.) 173229, India

Keywords:

haemagglutination antibody titre, delayed type hypersensitivity reaction, phagocytosis, nitric oxide, Candida albicans, flow cytometry

Abstract

In order to evaluate the role of ethyl acetate fraction (PB-EtAC) obtained from the Phyllostachys bambusoides leaves in the modulation of immune responses, detailed studies were carried out using a panel of in vivo assays. Oral administration of PB-EtAC (50–200 mg/Kg) stimulated the IgM and IgG titre expressed in the form of haemagglutination antibody (HA) titre. Further, it elicited a dose related increase in the delayed type hypersensitivity reaction (DTH) after 24 and 48 h in BALB/c mice. Besides augmenting the humoral and cell mediated immune response, the concentration of cytokines (IFN-γ, IL-2, and IL-4) in serum with respect to T cell interactions also increased significantly. It also induced macrophage phagocytosis, and nitric oxide (NO) production which resulted in a high degree of protection against Candida albicans and carbon clearance. Moreover, the enhancement in CD4 and CD8 cell populations as revealed by flow cytometry. Taken together this in vivo and ex vivo preclinical data, our results suggested that PB-EtAC acts as an effective immunostimulator eliciting both Th1 and Th2 immune responses. We are reporting first time the immunostimulatory potential of P. bambusoides and it might be regarded as a biological response modifier.

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Published

2014-02-17

How to Cite

Kumar, S., Sharma, G., Sidiq, T., Khajuria, A., Jain, M., Bhagwat, D., & Dhar, K. (2014). Immunomodulatory potential of a bioactive fraction from the leaves of Phyllostachys bambusoides (bamboo) in BALB/c mice. EXCLI Journal, 13, 137–150. Retrieved from https://www.excli.de/index.php/excli/article/view/688

Issue

Vol. 13 (2014)

Section

Original articles

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