Production of microbial transglutaminase by Streptoverticillium cinnamoneum KKP 1658

Authors

  • Vitaliy Kolotylo Department of Food Biotechnology and Microbiology, Institute of Food Sciences, Warsaw University of Life Sciences—SGGW, Nowoursynowska 159 C, 02-776 Warsaw, Poland https://orcid.org/0000-0001-5861-197X
  • Kamil Piwowarek Department of Food Biotechnology and Microbiology, Institute of Food Sciences, Warsaw University of Life Sciences—SGGW, Nowoursynowska 159 C, 02-776 Warsaw, Poland https://orcid.org/0000-0003-4203-4877
  • Marek Kieliszek Department of Food Biotechnology and Microbiology, Institute of Food Sciences, Warsaw University of Life Sciences— SGGW, Nowoursynowska 159 C, 02-776 Warsaw, Poland. E-mail: marek_kieliszek@sggw.edu.pl; marek-kieliszek@wp.pl https://orcid.org/0000-0002-5836-4865

DOI:

https://doi.org/10.17179/excli2024-7033

Keywords:

microbial transglutaminase, Streptoverticillium cinnamoneum, biosynthesis, waste products

Abstract

Transglutaminase finds broad applications in the food industry, influencing texture, shelf life and overall food quality. It can be utilized to create products with enhanced sensory and technological properties and serves as a tool to reduce food waste. The aim of this study was to optimize the production of microbial transglutaminase (MTG) by the genetically unmodified strain of Streptoverticillium cinnamoneum KKP 1658. Tryptone soy broth (TSB) was chosen as the optimal inoculation medium due to its high MTG activity in the cultivation substrate. The optimal inoculum incubation time was determined as 24 hours, with a dosage of 10 %. Various nitrogen sources were investigated while maintaining a consistent nitrogen dosage (0.2 %) (including aminobak, corn steep liquor, ammonium nitrate and ammonium sulphate) to achieve the highest microbiological transglutaminase activity. The combination of aminobak with corn steep liquor and a cultivation period of 72 hours (28 °C; pH 6.0-6.5) yielded the highest MTG activity at 6.59 U/mL.

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Published

2024-05-03

How to Cite

Kolotylo, V., Piwowarek, K., & Kieliszek, M. (2024). Production of microbial transglutaminase by Streptoverticillium cinnamoneum KKP 1658. EXCLI Journal, 23, 655–671. https://doi.org/10.17179/excli2024-7033

Issue

Vol. 23 (2024)

Section

Original articles

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Copyright (c) 2024 Vitaliy Kolotylo, Kamil Piwowarek, Marek Kieliszek

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