Unveiling the Ro60-Ro52 complex

Authors

  • Laura R. Rodríguez Present address: Stem Cells, Aging and Neurodegeneration Group, Department of Experimental Medical Science, Faculty of Medicine, Lund Stem Cell Center, Lund University, 22184, Lund, Sweden https://orcid.org/0000-0001-5290-2266
  • Jesus Vicente de Julián-Ortiz Molecular Topology and Drug Design Research Unit, Department of Physical Chemistry, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Valencia, Spain https://orcid.org/0000-0001-9403-8744
  • Fernando Rubio de la Rúa Department of Physical Chemistry, Faculty of Chemistry, University of Valencia, C/ Doctor Moliner 50, 46100, Burjassot, Spain https://orcid.org/0009-0009-3347-2673
  • Augusto Juste-Dolz Center for Research and Innovation on Bioengineering (Ci2B), Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain https://orcid.org/0000-0003-4889-6419
  • Ángel Maquieira Departamento de Química, Polytechnic University of Valencia, Camino de Vera s/n 46022, Valencia, Spain; Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022 Valencia, Spain https://orcid.org/0000-0003-4641-4957
  • Haydar Abdulhakim Mohammad-Salim Molecular Topology and Drug Design Research Unit, Department of Physical Chemistry, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Valencia, Spain; Faculty of Science, Department of Chemistry, University of Zakho, Zakho, Duhok 42001, Kurdistan Region, Iraq https://orcid.org/0000-0002-9600-3446
  • Sofiane Benmetir Molecular Topology and Drug Design Research Unit, Department of Physical Chemistry, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Valencia, Spain; Process and Environmental Engineering Laboratory (LIPE), Faculty of Chemistry, University of Science and Technology of Oran Mohamed BOUDIAF, P.O. Box 1503, El Mnaouer, 31000 Oran, Algeria https://orcid.org/0000-0002-2492-2750
  • Federico Vicente Pallardó Department of Physiology, Faculty of Medicine and Dentistry. University of Valencia-INCLIVA, 46010 Valencia, Spain; CIBER Rare Diseases (CIBERER), 46010 Valencia, Spain https://orcid.org/0000-0003-3715-1980
  • Pilar González-Cabo Department of Physiology, Faculty of Medicine and Dentistry. University of Valencia-INCLIVA, 46010 Valencia, Spain. E-mail: Pilar.Gonzalez-Cabo@uv.es https://orcid.org/0000-0003-3131-6334
  • David Gimenez-Romero Department of Physical Chemistry, Faculty of Chemistry, University of Valencia, C/ Doctor Moliner 50, 46100, Burjassot, Spain. E-mail: giroda@uv.es https://orcid.org/0000-0001-6489-9308

DOI:

https://doi.org/10.17179/excli2024-7141

Keywords:

Ro52/Trim21, Ro60/Trove2, transient complex, QCM-D, PLA, IIF

Abstract

The coexistence within a subcellular complex of inter-cellular proteins Ro60, responsible for preserving ncRNA quality, and Ro52, involved in intracellular proteolysis, has been a subject of ongoing debate. Employing molecular docking in tandem with experimental methods like Quartz Crystal Microbalance with Dissipation (QCM-D), Proximity Ligation Assay (PLA), and Indirect Immunofluorescence (IIF), we reveal the presence of Ro60 associating with Ro52 within the cytoplasm. This result unveils the formation of a weak transient complex with a Ka ≈ (3.7 ± 0.3) x 106 M-1, where the toroid-shaped Ro60 structure interacts with the Ro52’s Fc receptor, aligning horizontally within the PRY-SPRY domains of the Ro52’s homodimer. The stability of this complex relies on the interaction between Ro52 chain A and specific Ro60 residues, such as K133, W177, or L185, vital in the Ro60-YRNA bond. These findings bridge the role of Ro60 in YRNA management with Ro52's function in intracellular proteolysis, emphasizing the potential impact of transient complexes on cellular pathways.

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Published

2024-06-10

How to Cite

Rodríguez, L. R., Vicente de Julián-Ortiz, J., Rubio de la Rúa, F., Juste-Dolz, A., Maquieira, Ángel, Mohammad-Salim, H. A., … Gimenez-Romero, D. (2024). Unveiling the Ro60-Ro52 complex. EXCLI Journal, 23, 888–903. https://doi.org/10.17179/excli2024-7141

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