Treatment with platelet lysate induces endothelial differentation of bone marrow mesenchymal stem cells under fluid shear stress

Authors

  • Farshad Homayouni Moghadam Shahid Sadoughi University of Medical Sciences, Department of Physiology, School of Medicine, Yazd, Iran; Shahid Sadoughi University of Medical Sciences, Neurobiomedical Research Center, School of Medicine, Yazd, Iran; Yazd Cardiovascular Research Center
  • Tahereh Tayebi Shahid Sadoughi University of Medical Sciences, Department of Physiology, School of Medicine, Yazd, Iran
  • Alireza Moradi Shahid Sadoughi University of Medical Sciences, Department of Medicinal Chemistry, School of Pharmacy, Yazd, Iran
  • Hamid Nadri Shahid Sadoughi University of Medical Sciences, Department of Medicinal Chemistry, School of Pharmacy, Yazd, Iran
  • Kazem Barzegar Shahid Sadoughi University of Medical Sciences, English Language Department, School of Medicine, Yazd, Iran
  • Gilda Eslami Shahid Sadoughi University of Medical Sciences, Department of Medical Parasitology and Mycology, School of Medicine, Yazd, Iran

Keywords:

endothelial cell, differentiation, platelet lysate, fluid shear stress, mesenchymal stem cell

Abstract

By considering stem cell-based therapies as a new hope for the treatment of some tragic diseases, marrow stromal cells or marrow mesenchymal stem cells (MSCs) were considered as a suitable and safe multipotential cell source for this new therapeutic approach. For this purpose, many investigations have been performed on differentiation of MSCs toward specific cell lines to overcome the demand for providing the organ specific cells for cell therapy or preparation of engineered tissues. In the present study, differentiation of MSCs to endothelial cells (ECs) by mechanical and chemical stimulation was evaluated. Fluid shear stress (FSS) was used as mechanical inducer, while platelet lysate (PL) and estradiol (E) were used as chemical induction factors. MSCs were placed under FSS with different forces (2, 5 and 10dyn/cm2) for different periods (6, 12 and 24 hours). In some groups, PL and E were added to the culture media to evaluate their effect on expression of EC specific markers. This investigation revealed that FSS with low tension (2.5-5 dyn/cm2) for a long time (24 hours) or high tension (10 dyn/cm2) in short time (6 hours) in the presence of PL could differentiate MSCs toward ECs. The presence of PL was necessary for initiation of endothelial differentiation, and in the absence of PL, there was not any expression of CD34 and Cadherin5 (Cdh5) among cells. Adding E to the culture medium did not change the rate of endothelial differentiation under FSS. Generated endothelial progenitors could produce von Willebrand factor (vWF) after two weeks culture and also they formed tubular structures after culture on matrigel.

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Published

2014-06-03

How to Cite

Homayouni Moghadam, F., Tayebi, T., Moradi, A., Nadri, H., Barzegar, K., & Eslami, G. (2014). Treatment with platelet lysate induces endothelial differentation of bone marrow mesenchymal stem cells under fluid shear stress. EXCLI Journal, 13, 638–649. Retrieved from https://www.excli.de/index.php/excli/article/view/728

Issue

Vol. 13 (2014)

Section

Original articles

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