Sodium valproate enhances urethane tumorigenicity in lungs of male but not female mice

Authors

  • Donatas Stakisaitis Laboratory of Carcinogenesis and Tumor Pathophysiology, Institute of Oncology, Vilnius University, Vilnius, Lithuania; Mykolas Romeris University, Vilnius, Lithuania
  • Saule Uleckiene Laboratory of Carcinogenesis and Tumor Pathophysiology, Institute of Oncology, Vilnius University, Vilnius, Lithuania
  • Janina Didziapetriene Laboratory of Carcinogenesis and Tumor Pathophysiology, Institute of Oncology, Vilnius University, Vilnius, Lithuania
  • Angelija Valanciute Lithuanian University of Health Sciences, Department of Histology and Embryology, Kaunas, Lithuania
  • Raminta Mozuraite Lithuanian University of Health Sciences, Department of Histology and Embryology, Kaunas, Lithuania
  • Paulius Matusevicius Lithuanian University of Health Sciences, Veterinary Academy, Kaunas, Lithuania

Keywords:

sodium valproate, urethane, mice, lung tumorigenesis, gender differences

Abstract

In the study, the possible effect of sodium valproate (NaVP) on urethane-induced lung tumors in mice has been evaluated. BALB/c mice (n = 120; 4–6 weeks old, both sexes) were used in the following groups: 1) urethane-treated, 2) urethane–NaVP-treated, 3) only NaVP-treated, 4) control. In the same groups, castrated male mice (n = 48) were investigated. Urethane was given by intraperitoneal injections 10 mg/mouse, twice a week, the total dose 50 mg/mouse. In NaVP-treated mice, the 0.4 % NaVP aqueous solution was offered to mice ad libitum. The duration of the experiment was 6 months. The number of tumors per mouse in urethane–NaVP-treated males was significantly higher than in those treated with urethane only (13.82 ± 1.12 vs 6.77 ± 0.43, p < 0.0001). No significant difference in the number of tumors per mouse was revealed while comparing the female urethane- and urethane–NaVP-treated groups (6.50 ± 0.79 vs 8.15 ± 0.55, p = 0.105). No difference in the number of tumors per mouse was found in urethane–NaVP-treated castrated males as compared with urethane-treated castrated males. However, in the urethane–NaVP-treated castrated males the number of tumors per mouse was significantly lower than in analogous non-castrated males (7.8 ± 1.67 vs 13.82 ± 1.12, p < 0.01). NaVP combined with urethane potentiates urethane tumorigenicity in BALB/c non-castrated but not in female and castrated male mice. These data indicate an important role of testosterone in the urethane-NaVP induced lung tumorigenesis.

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Published

2014-06-05

How to Cite

Stakisaitis, D., Uleckiene, S., Didziapetriene, J., Valanciute, A., Mozuraite, R., & Matusevicius, P. (2014). Sodium valproate enhances urethane tumorigenicity in lungs of male but not female mice. EXCLI Journal, 13, 667–687. Retrieved from https://www.excli.de/excli/article/view/730

Issue

Vol. 13 (2014)

Section

Original articles

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