Induction of spinal long-term synaptic potentiation is sensitive to inhibition of neuronal NOS in L5 spinal nerve-transected rats

Authors

  • Zahra Bahari Department of Neurophysiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Homa Manaheji Department of Physiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Narges Hosseinmardi Department of Neurophysiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Gholam Hossein Meftahi Neuroscience Research Center, Baqiyatallah (a.s.) University of Medical Sciences, Tehran, Iran
  • Mehdi Sadeghi Department of Physiology, Bushehr University of Medical Sciences, Bushehr, Iran
  • Samira Danialy Department of Neurophysiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Seyed Mohammad Noorbakhsh Science Beam CO., Tehran, Iran

Keywords:

neuropathic pain, nNOS, LTP, dorsal horn

Abstract

The role of neuronal nitric oxide synthase (nNOS) in the central mechanism of neuropathic pain and long-term potentiation (LTP) of peripheral afferents remains obscure. The current study investigated the effect of intrathecal application of 7-nitroindazole (7-NI), a selective nNOS inhibitor (8.15 µg/5µl), on mechanical allodynia on day 14 after L5 spinal nerve transection. Furthermore, using in vivo single unit extracellular recording, we examined the effect of 7-NI on the induction of LTP of Aδ- and C-fiber-evoked responses. We have demonstrated that 7-NI attenuates nerve-injury-evoked mechanical allodynia. Additionally, our electrophysiological study has shown that the spinal administration of 7-NI significantly inhibits the induction of the LTP of Aδ- and C-fiber-evoked responses on day 14 after neuropathy. These data suggest that activation of nNOS may be crucial for the induction of the spinal LTP of Aδ- and C-fiber-evoked responses following peripheral nerve damage.

Published

2014-07-14

How to Cite

Bahari, Z., Manaheji, H., Hosseinmardi, N., Meftahi, G. H., Sadeghi, M., Danialy, S., & Noorbakhsh, S. M. (2014). Induction of spinal long-term synaptic potentiation is sensitive to inhibition of neuronal NOS in L5 spinal nerve-transected rats. EXCLI Journal, 13, 751–760. Retrieved from https://www.excli.de/index.php/excli/article/view/737

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Section

Original articles

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