Biological activities and chemical composition of lichens from Serbia


  • Marijana Kosanic Department of Biology, Faculty of Science, University of Kragujevac, 34000 Kragujevac, Serbia
  • Branislav Rankovic Department of Biology, Faculty of Science, University of Kragujevac, 34000 Kragujevac, Serbia
  • Tatjana Stanojkovic Institute of Oncology and Radiology of Serbia, 11000 Belgrade, Serbia
  • Perica Vasiljevic Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, 18000 Niš, Serbia
  • Nedeljko Manojlovic Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia


HPLC analysis, biological activities, lichens, lichens compound


The aim of this study is to investigate chemical composition of acetone extracts of the lichens Parmelia arseneana and Acarospora fuscata and in vitro antioxidant, antimicrobial, and anticancer activities of these extracts and gyrophoric acid isolated from A. fuscata. The HPLC-UV method was used for the identification of secondary metabolites. Stictic acid, norstictic acid, gyrophoric acid, usnic acid, atranorin and chloroatranorin were identified in the A. fuscata. In P. arseneana, we detected stictic acid, norstictic acid, usnic acid and atranorin, while gyrophoric acid was not identified. Antioxidant activity was evaluated by measuring the scavenging capacity of tested samples on DPPH and superoxide anion radicals, reducing the power of samples and determination of total phenolic compounds in extracts. As a result of the study, gyrophoric acid was found to have the largest DPPH radical scavenging activity with an IC50 value of 105.75 µg/ml. Moreover, the tested samples had an effective superoxide anion radical scavenging and reducing power. The total content of phenol in extracts was determined as pyrocatechol equivalent. The antimicrobial activity was estimated by determination of the minimal inhibitory concentration by the broth microdilution method. The most active was also gyrophoric acid, with minimum inhibitory concentration values ranging from 0.019 to 1.25 mg/ml. Anticancer activity was tested against LS174 (human colon carcinoma cell line), A549 (human lung carcinoma cell line), Fem-x (malignant melanoma cell line), and a chronic myelogeneous leukaemia K562 cell line using the MTT method. Extract of P. arseneana expressed the strongest anticancer activity against all cell lines with IC50 values ranging from 11.61 to 47.06 µg/ml.



How to Cite

Kosanic, M., Rankovic, B., Stanojkovic, T., Vasiljevic, P., & Manojlovic, N. (2014). Biological activities and chemical composition of lichens from Serbia. EXCLI Journal, 13, 1226–1238. Retrieved from



Original articles