Drosophila melanogaster - an embryonic model for studying behavioral and biochemical effects of manganese exposure

Authors

  • Ana Paula Lausmann Ternes Centro Interdisciplinar de Pesquisa em Biotecnologia (CIP/BIOTEC), Universidade Federal do Pampa, Campus São Gabriel, RS, 97300 000, Brasil
  • Ana Paula Zemolin Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brasil
  • Litiele Cezar da Cruz Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brasil
  • Gustavo Felipe da Silva Centro Interdisciplinar de Pesquisa em Biotecnologia (CIP/BIOTEC), Universidade Federal do Pampa, Campus São Gabriel, RS, 97300 000, Brasil
  • Ana Paula Fleig Saidelles Centro Interdisciplinar de Pesquisa em Biotecnologia (CIP/BIOTEC), Universidade Federal do Pampa, Campus São Gabriel, RS, 97300 000, Brasil
  • Mariane Trindade de Paula Programa de Pós Graduação em Bioquímica, Universidade Federal do Pampa, Campus Uruguaiana, CEP 97500-970
  • Caroline Wagner Universidade Federal do Pampa, Campus Caçapava do Sul, RS, CEP 96570-000 Brasil
  • Ronaldo Medeiros Golombieski Laboratório de Biologia Molecular de Drosophila e Sequenciamento (LabDros), Universidade Federal de Santa Maria, Santa Maria, RS, 97300 000
  • Érico Marlon de Moraes Flores Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brasil
  • Rochele Sogari Picoloto Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brasil
  • Antônio Batista Pereira Centro Interdisciplinar de Pesquisa em Biotecnologia (CIP/BIOTEC), Universidade Federal do Pampa, Campus São Gabriel, RS, 97300 000, Brasil
  • Jeferson Luis Franco Centro Interdisciplinar de Pesquisa em Biotecnologia (CIP/BIOTEC), Universidade Federal do Pampa, Campus São Gabriel, RS, 97300 000, Brasil
  • Thaís Posser Centro Interdisciplinar de Pesquisa em Biotecnologia (CIP/BIOTEC), Universidade Federal do Pampa, Campus São Gabriel, RS, 97300 000, Brasil

Keywords:

manganese, Drosophila melanogaster, MAPK, oxidative stress, Thioredoxin reductase

Abstract

Embryonic animals are especially susceptible to metal exposure. Manganese (Mn) is an essential element, but in excess it can induce toxicity. In this study we used Drosophila melanogaster as an embryonic model to investigate biochemical and behavioral alterations due to Mn exposure. Flies were treated with standard medium supplemented with MnCl2 at 0.1 mM, 0.5 mM or 1 mM from the egg to the adult stage. At 0.5 mM and 1 mM Mn, newly ecloded flies showed significantly enhanced locomotor activity when assessed by negative geotaxis behavior. In addition, a significant increase in Mn levels (p < 0.0001) was observed, while Ca, Fe, Cu, Zn and S levels were significantly decreased. A significant drop in cell viability occurred in flies exposed to 1 mM Mn. There was also an induction of reactive oxygen species at 0.5 mM and 1 mM Mn (p < 0.05). At 1 mM, Mn increased Catalase (p < 0.005), Superoxide Dismutase (p < 0.005) and Hsp83 (p < 0.0001) mRNA expression, without altering Catalase or Superoxide Dismutase activity; the activity of Thioredoxin reductase and Glutatione-S-transferase enzymes was increased. Mn treatment did not alter ERK or JNK1/2 phosphorylation, but at 1 mM caused an inhibition of p38MAPK phosphorylation. Together these data suggest mechanisms of adaptation in the fly response to Mn exposure in embryonic life.

Published

2014-11-21

How to Cite

Ternes, A. P. L., Zemolin, A. P., da Cruz, L. C., da Silva, G. F., Saidelles, A. P. F., de Paula, M. T., … Posser, T. (2014). Drosophila melanogaster - an embryonic model for studying behavioral and biochemical effects of manganese exposure. EXCLI Journal, 13, 1239–1253. Retrieved from https://www.excli.de/index.php/excli/article/view/776

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