Seromolecular assess of Toxoplasma gondii infection in pregnant women and neonatal umbilical cord blood

Authors

  • Mohammad Menati Rashno Department of Biotechnology, Damghan Branch, Islamic Azad University, Damghan, Iran. Tel: +98-9384409404, E-mail: mohammadbiotech3@gmail.com
  • Shirzad Fallahi Department of Medical Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Zahra Arab-Mazar Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medial Sciences, Tehran, Iran
  • Hassan Dana Department of Biotechnology, Damghan Branch, Islamic Azad University, Damghan, Iran

DOI:

https://doi.org/10.17179/excli2018-1510

Keywords:

pregnant women, cord blood, serological methods, molecular methods

Abstract

Toxoplasmosis is considered as one of the most prevalent human parasitic infections that can be transmitted from mother to the fetus. The onset of toxoplasmosis during pregnancy has clinical complications including spontaneous abortion, preterm labor, stillbirth and fetal abnormalities. The aim of this study was to investigate the prevalence of Toxoplasmosis infection in pregnant women and their infants in Lorestan province, Western Iran. Blood and sera samples were collected from 98 pregnant women and their infants. All collected samples were examined for Toxoplasma gondii infection by serological tests (ELISA IgM & IgG) and PCR assay. Among the 98 samples of mother and umbilical cord prevalence of anti-Toxoplasma IgG, was 34/98 (34.69 %) and 33/98 (33.67 %), respectively. All pregnant women were negative for, anti-Toxoplasma IgM while it was found in 5/98 (5.1 %) of umbilical cords. Based on PCR analysis, Toxoplasma infection was detected in 5 (5.1 %) and 7 (7.14 %) of mother and umbilical cords, respectively. Molecular test along with evaluation of IgM (P <0.001) and IgG (P = 0.001) were significantly correlated.

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Published

2019-01-02

How to Cite

Rashno, M. M., Fallahi, S., Arab-Mazar, Z., & Dana, H. (2019). Seromolecular assess of Toxoplasma gondii infection in pregnant women and neonatal umbilical cord blood. EXCLI Journal, 18, 1–7. https://doi.org/10.17179/excli2018-1510

Issue

Vol. 18 (2019)

Section

Original articles

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