Therapeutic effect of Baccharis anomala DC. extracts on activated hepatic stellate cells

Authors

  • Bruno de Souza Basso PUCRS, Escola de Ciências, Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul, Av. Ipiranga, 6681, Sala 221, bloco C, prédio 12, Porto Alegre, RS, Brazil. Tel.: 00+55+51 3320.4147; Fax: 00+55+51 3320.3500
  • Fernanda Cristina de Mesquita PUCRS, Escola de Ciências, Laboratório de Pesquisa em Biofísica Celular e Inflamação, Porto Alegre, Brazil; Graduate Program in Neuroengineering, Edmond and Lily Safra International Institute of Neuroscience, Santos Dumont Institute, Macaiba, Brazil
  • Henrique Bregolin Dias PUCRS, Escola de Ciências, Laboratório de Pesquisa em Biofísica Celular e Inflamação, Porto Alegre, Brazil
  • Gabriele Catyana Krause PUCRS, Escola de Ciências, Laboratório de Pesquisa em Biofísica Celular e Inflamação, Porto Alegre, Brazilk
  • Matheus Scherer PUCRS, Escola de Ciências, Laboratório de Pesquisa em Biofísica Celular e Inflamação, Porto Alegre, Brazil
  • Eliane Romanato Santarém PUCRS, Escola de Ciências, Laboratório de Biotecnologia Vegetal, Porto Alegre, Brazil
  • Jarbas Rodrigues de Oliveira PUCRS, Escola de Ciências, Laboratório de Pesquisa em Biofísica Celular e Inflamação, Porto Alegre, Brazil

DOI:

https://doi.org/10.17179/excli2018-1696

Keywords:

hepatic fibrosis, HSCs, Baccharis, PPAR-γ, phenolic compounds

Abstract

The therapeutic potential of Baccharis anomala DC. extracts was evaluated through its cytotoxic and antiproliferative effect and their phenotypic reversion property in activated hepatic stellate cells (HSCs). Baccharis anomala is distributed in Brazil (southeastern and south regions) and used for diuretic effect in folk medicine. Four fractions were obtained from the fractionation of the methanolic extract. Fractions III and IV decreased cell proliferation without increasing cell necrosis markers levels and induced cell cycle arrest in G1 phase. Fraction III induced phenotypic reversion through PPAR-γ activation pathway, while fraction IV did not alter PPAR-α/γ expression levels, suggesting that there is an independent PPAR-α/γ pathway involved. Hydroxybenzoic, chlorogenic and coumaric acids were identified. Fractions III and IV showed antiproliferative effect and ability to induce reversion of activated phenotype of HSCs.

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Published

2019-02-18

How to Cite

Basso, B. de S., de Mesquita, F. C., Dias, H. B., Krause, G. C., Scherer, M., Santarém, E. R., & de Oliveira, J. R. (2019). Therapeutic effect of Baccharis anomala DC. extracts on activated hepatic stellate cells. EXCLI Journal, 18, 91–105. https://doi.org/10.17179/excli2018-1696

Issue

Vol. 18 (2019)

Section

Original articles

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