@article{Hosseini_Shirvani_Aghaei_Arabzadeh_Hofmeister_2022, title={Ameliorative effects of high intensity interval training and Lactobacillus rhamnosus GG against tetracycline-induced fatty liver in rats: a gene expression profiling comparative study}, volume={21}, url={https://www.excli.de/index.php/excli/article/view/4791}, DOI={10.17179/excli2022-4791}, abstractNote={<p class="Abstract"><span lang="EN-US">Exercise training and probiotics have been suggested as a treatment for the prevention of chronic liver damage such as non-alcoholic fatty liver disease (NAFLD). <em>Lactobacillus rhamnosus Gorbach‐Goldin (LGG)</em> is one of the most widely used probiotic strains that decreases liver damage. Thus, this study aims to consider the ameliorative effects of high intensity interval training (HIIT) and <em>LGG</em> against tetracycline-induced fatty liver in rats. Eighty male Wistar rats were randomly divided into 8 groups of (n=10 each group): control, LGG, HIIT, LGG+HIIT, NAFLD, NAFLD+LGG, NAFLD+HIIT, and NAFLD+LGG+HIIT. The rats are treated by intraperitoneal injection with 140 mg/kg<sup>−1</sup> tetracycline, an antibiotic previously known to induce steatosis. The exercise training groups performed HIIT 5 days/week for 5 weeks. 10<sup>7</sup> colony-forming units (cfu) of LGG were gavaged for LGG groups 5 days/week for 5 weeks. Probiotic supplementation in combination with interval training significantly decreased <em>tissue inhibitor of matrix metalloproteinases-1 (TIMP-1)</em> mRNA and <em>matrix metalloproteinase-2</em> <em>(MMP-2)</em> mRNA in the liver (p<0.05), while the levels of <em>lysosomal acid lipase</em> <em>(LIPA)</em> mRNA was significantly increased compared to NAFLD group. Also, compared with NAFLD group, NAFLD+LGG, NAFLD+HIIT and NAFLD+LGG+HIIT groups showed a significant decrease in hepatic <em>monocyte chemoattractant protein-1</em> <em>(MCP-1)</em>. Compared to LGG and LGG+HIIT groups, all NAFLD groups showed a significant decrease in <em>apolipoprotein C3 (APOC3)</em> in liver tissue (p<0.05). The results suggested that interval exercise with LGG supplementation minimizes cell destruction and inflammation in liver tissue due to NAFLD by improving gene expression profiles.</span></p>}, journal={EXCLI Journal}, author={Hosseini, Hamideh Mahmoodzadeh and Shirvani, Hossein and Aghaei, Fariba and Arabzadeh, Ehsan and Hofmeister, Martin}, year={2022}, month={Jul.}, pages={991–1006} }