@article{Farías-Sánchez_López-Miranda_Castro-Montoya_Saucedo-Luna_Carrillo-Parra_López-Albarrán_Pineda-Pimentel_Rutiaga-Quiñones_2015, title={Comparison of five pretreatments for the production of fermentable sugars obtained from Pinus pseudostrobus L. wood}, volume={14}, url={https://www.excli.de/index.php/excli/article/view/553}, DOI={10.17179/excli2014-613}, abstractNote={<p>To benefit from the use of a waste product such as pine sawdust from a sawmill in Michoacán, Mexico, five different pretreatments for the production of reducing sugars by enzymatic hydrolysis were evaluated (sodium hydroxide, sulfuric acid, steam explosion, organosolv and combined method nitric acid / sodium hydroxide). The main finding of the study was that the pretreatment with 6 % HNO<sub>3</sub> and 1 % NaOH led to better yields than those obtained with sodium hydroxide, dilute sulfuric acid, steam explosion, and organosolv pretreatments. Also, HNO<sub>3</sub> yields were maximized by the factorial method. With those results the maxima concentration of reducing sugar found was 97.83 ± 1.59, obtained after pretreatment with 7.5 % HNO<sub>3</sub> at 120 °C for 30 minutes; followed by 1 % of NaOH at 90 °C for 30 minutes at pH 4.5 for 168 hours with a load enzyme of 25 FPU/g of total carbohydrates. Comparing the results obtained by the authors with those reported in the literature, the combined method was found to be suitable for use in the exploitation of sawdust. </p>}, journal={EXCLI Journal}, author={Farías-Sánchez, Juan Carlos and López-Miranda, Javier and Castro-Montoya, Agustín Jaime and Saucedo-Luna, Jaime and Carrillo-Parra, Artemio and López-Albarrán, Pablo and Pineda-Pimentel, María Guadalupe and Rutiaga-Quiñones, José Guadalupe}, year={2015}, month={Mar.}, pages={430–438} }