TY - JOUR AU - Kazemian, Shakiba AU - Ahmadi, Reza AU - Ferns, Gordon A. AU - Rafiei, Ali AU - Azadegan-Dehkordi, Fatemeh AU - Khaledifar, Arsalan AU - Mohammad-Rezaei, Mina AU - Bagheri, Nader PY - 2022/09/08 Y2 - 2024/03/28 TI - Correlation of miR-24-3p and miR-595 expression with CCL3, CCL4, IL1-β, TNFαIP3, and NF-κBIα genes in PBMCs of patients with coronary artery disease JF - EXCLI Journal JA - EXCLI J. VL - 21 IS - SE - Original articles DO - 10.17179/excli2022-5266 UR - https://www.excli.de/index.php/excli/article/view/5266 SP - 1184-1195 AB - <p>Inflammation has been well recognized to play an important role in developing coronary artery disease (CAD). By regulating essential genes in this pathway post-transcriptionally, MicroRNAs (miRNAs) may help or hinder the development of atherosclerotic lesions. The aim of this study was to investigate the expression of miR-24-3p, miR-595, <em>CCL3</em>, <em>CCL4</em>, <em>IL-1β</em>, <em>TNFαIP3</em>, and <em>NF-κBIα</em> in the peripheral blood mononuclear cells (PBMCs) of CAD and control groups and to examine whether any correlation exists between the expression of miRs and genes in CAD group. A total of 168 subjects (84 CAD subjects and 84 control subjects) were examined in this research. Expression levels of miR-24-3p, miR-595, CCL3, CCL4, IL-1β<em>, TNFαIP3</em>, and <em>NF-κBIα</em> in PBMCs were measured using the real-time PCR technique. A comparison of the CAD group with the control group indicated significantly increased expression levels of <em>CCL3</em>, <em>CCL4,</em> and <em>IL-1β </em>(Fold Change (FC)=4, <em>P</em>=0.009; FC=2.9, <em>P</em>=0.01; FC=1.8, <em>P</em>=0.019, respectively) and remarkably reduced expression levels of <em>TNFαIP3</em> and <em>NF-κBIα </em>(FC=-1.4, <em>P</em>=0.03 and FC=-5.9, <em>P</em>=0.001, respectively). Moreover, the expression levels of miR-24-3p downregulated (FC=-2.5, <em>P</em>=0.005) and miR-595 upregulated (FC=1.9, <em>P</em>=0.009) in the CAD group. There was a statistical correlation between the number of clogged arteries with expression levels of miR-24-3p, miR-595, <em>CCL3</em>, <em>CCL4</em>, <em>IL-1β</em>, <em>TNFαIP3</em>, and <em>NF-κBIα</em> in the CAD group. Also, there was a statistical correlation between expression levels of miR-24-3p and miR-595 with <em>CCL3</em>, <em>CCL4</em>, <em>IL-1β</em>, <em>TNFαIP3</em>, and <em>NF-κBIα</em> gene expression in the CAD group. In CAD patients, decreased expression of miR-24-3p and increased expression of miR-595 may aid the progression of atherosclerotic plaques by regulating <em>CCL3</em>, <em>CCL4</em>, <em>IL-1β</em>, <em>TNFαIP3</em>, and <em>NF-κBIα</em> gene expression.</p> ER -