TY - JOUR AU - Sayed, Waiel F. AU - Salem, Wesam M. A. AU - Haridy, Mohie A. M. AU - Hassan, Ne'mat H. PY - 2015/06/16 Y2 - 2024/03/29 TI - Efficacy of Caltropis procera and Ficus sycomorus extracts in treating MRSA (methicillin-resistant Staphylococcus aureus)-keratitis in rabbit JF - EXCLI Journal JA - EXCLI J. VL - 14 IS - 0 SE - Original articles DO - 10.17179/excli2015-350 UR - https://www.excli.de/index.php/excli/article/view/579 SP - 747-757 AB - <p>MRSA-induced keratitis in rabbit was used to evaluate the therapeutic effect of <i>F. sycomorus</i> leaves and <i>C. procera</i> latex extracts. Within the 6 rabbit groups tested, group 1 received sterilized saline, while other groups (2 to 6) received 100 μl of intrastromal injections of 1.5×10<sup>3</sup> colony forming unit (cfu) ml<sup>-1</sup> of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). After 12 hours, groups 3 to 6 also received chloramphenicol, aqueous extract of <i>C. procera</i> latex, aqueous and alcoholic extracts of <i>F. sycomorus</i> leaves, respectively 3 times daily for 12 successive days. The tested extracts inhibited MRSA growth <i>in vitro</i> (i.e. on culture medium). Colony counts in cornea discs from groups 3 to 6 were significantly reduced (<i>P </i>≤ 0.001) compared to group 2 (untreated). Clinical signs of keratitis were observed on group 2 until the end of experiment. In groups 3 to 6, gradual recovery was observed and signs disappeared by the 12<sup>th</sup> DPI (days post inoculation). Only mild symptoms persisted in group 5 (aqueous extract of leaves). In group 3 and 5, cornea, iris, ciliary body and conjunctiva showed mild leukocytic infiltration and depigmentation of melanin cells while recovery of cornea and iris was observed in groups 4 and 6. In conclusion, the used extracts have potential therapeutic effects on MRSA-induced keratitis in rabbit.</p> ER -