EXCLI Journal https://www.excli.de/index.php/excli <center><img class="img-responsive" src="/public/site/images/lindemann/Lucida_logo_neu_geschrieben.PNG" alt="EXCLI Journal Logo"></center> <p>EXCLI Journal publishes original research reports, authoritative reviews and case reports of experimental and clinical sciences.</p> <div class="toggleBox"><input id="toggleContent" name="toggleContent" type="checkbox"> <label class="open" for="toggleContent">More...</label> <label class="close" for="toggleContent">Less...</label> <div> <p><strong>EXCLI Journal </strong>(eISSN 1611-2156)&nbsp;is particularly keen to keep a broad view of science and technology, and therefore welcomes papers which bridge disciplines and may not suit the narrow specialism of other journals. Although the general emphasis is on biological sciences, studies from the following fields are explicitly encouraged:</p> <p>Immunology, toxicology, ergonomics, neurosciences, psychology, occupational medicine, clinical and preclinical studies, drug development, pharmacology, environmental health, chemistry including analytical chemistry, biochemistry, cell biology, genetics, forensic medicine, oncology and cancer research, proteomics, systems biology, hepatology and gastroenterology, aging research, psychiatric research, behavioral sciences.</p> </div> </div> en-US <p>Authors who publish in this journal agree to the following terms:</p> <ul> <li>The authors keep the copyright and grant the journal the right of first publication under the terms of the Creative Commons Attribution license, <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" rel="noopener">CC BY 4.0</a>. This licencse permits unrestricted use, distribution and reproduction in any medium, provided that the original work is properly cited.</li> <li>The use of general descriptive names, trade names, trademarks, and so forth in this publication, even if not specifically identified, does not imply that these names are not protected by the relevant laws and regulations.</li> <li>Because the advice and information in this journal are believed to be true and accurate at the time of publication, neither the authors, the editors, nor the publisher accept any legal responsibility for any errors or omissions presented in the publication. The publisher makes no guarantee, express or implied, with respect to the material contained herein.</li> <li>The authors can enter into additional contracts for the non-exclusive distribution of the journal's published version by citing the initial publication in this journal (e.g. publishing in an institutional repository or in a book).</li> </ul> excli@ifado.de (EXCLI Journal) excli@ifado.de (Webmaster) Mon, 04 Jan 2021 08:22:24 +0100 OJS 3.2.1.1 http://blogs.law.harvard.edu/tech/rss 60 The ınvestıgatıon of effect of the Klotho Proteın on the expressıon of NHEJ Repaır Pathway Genes Ku70 Ku80 and DNA-PK and on the workıng of theır proteıns https://www.excli.de/index.php/excli/article/view/3443 <p>Aging is a process in which the balance of the organism's relations with the environment over time results in favor of the environment. The rate of progress of this process depends on the interaction of the organism with the environment and its inherited characteristics. In this study the Klotho (Kl) gene, which is one of the genetic factors associated with aging was discussed. The Klotho gene, which first described in the mice located in the q-arm of chromosome 13 in the human, and encodes the type 1 single-pass transmembrane glycoprotein located in the Golgi apparatus. Athough there are some studies suggesting how Klotho affects intracellular molecular mechanisms, the role of this protein on the DNA double strand brekage (DSB) repair mechanisms, which are also known to have an effect on the aging process, cannot be elucidate yet. In this study, we aimed to investigate how Klotho affects the expression of some genes, especially known to work in the non-homologous end joint (NHEJ) repair, one of the repair mechanisms in the DNA DSB repair.<br>In the study, the mouse hippocampal cell lines HT22 cells were used. First, the IC50 dose of Endosulfan, which is known to produce double-chain fracture in DNA, was determined. Then, using this dose the HT22 cells were treated in different experimental protocols, and finally RNA and protein isolation was performed in those cells. From obtained total RNAs the cDNAs were generated, and then changes in the expressions of the Ku70, Ku80 and DNA-PK genes were determined by RT-PCR. Changes in protein level were determined by Western Blotting methods. The results showed that the expression levels of studied genes increased significantly only in Endosulfan-treated cells, but was not increased in the cells which also treated with Klotho protein. These results suggest that Klotho minimizes the harmful effect of Endosulfan and suppresses the NHEJ pathway. Thus, for the first time in the literature, the role of Klotho protein in the NHEJ repair pathway was investigated. <br>Key Words: Aging, Klotho, NHEJ, HT22 and Endosulfan</p> Sibel Demir Öztürk, Sadegül Tuncer, Hüsniye Rüstemoğlu, Aydın Rüstemoğlu Copyright (c) https://www.excli.de/index.php/excli/article/view/3443 Incidence Investigation of Accidents in Chemical Industries: A Comprehensive Study based on Factor Analysis https://www.excli.de/index.php/excli/article/view/3441 <p><strong>Introduction:</strong> Modelling and analysing accidents in chemical process industries (through the path of accident analysis) can contribute to designing safety programs for hazard prevention and control. This study aimed to identify factors affecting the incidence of chemical accidents.</p> <p><strong>Method:</strong> The present study was an analytical study that investigated 840 accidents in 42 chemical process industries over 11 years (2008-2018). Data analysis was conducted using exploratory and confirmatory factor analysis (EFA &amp; CFA), and structural equation modelling (SEM). Data analysis was done using IBM SPSS AMOS v. 22. Moreover, χ2/df, RMSEA, CFI, NFI and NNFA (TLI) indices were used as model fit indices.</p> <p><strong>Results:</strong> The descriptive results showed that 45.3% and 32.14% of the accidents were related to transportation and release of chemicals, respectively. Factor analysis showed that 6 latent factors and 37 indicator variables affected the accidents. SEM findings showed that latent factors including individual &amp; occupational, training, risk management and their indicator variables had indirect effects on the chemical accidents (p&lt;0.05), while unsafe conditions and unsafe acts latent factors with their indicator variables had direct effects on the incidence of chemical accidents (p&lt;0.05).</p> <p><strong>Conclusion:</strong> The findings confirmed that the chemical accidents are affected by different causal layers.</p> Ahmad Soltanzadeh, Mohsen Sadeghi Yarandi, Shahram Arsang-Jang, Mohsen Mahdinia, Heidar mohammadi Copyright (c) https://www.excli.de/index.php/excli/article/view/3441 The THE EFECT OF CIGARETTE SMOKE CANDENSATE ON MOUSE EMBERYO DEVELOPMENT AND EXPRESSION OF PLURIPOTENCY AND APOPTPTIC GENES IN VITRO https://www.excli.de/index.php/excli/article/view/3440 <p>It has been reported that cigarette smoke has adverse effects on the reproductive process. The aim of the present study was to investigate the effect of cigarette smoke condensate (CSC) &nbsp;on in vitro development of mouse embryo. Three thousand NMRI mice 2PN embryos were divided into six groups (n=500). The test group were exposed to 20, 40, 80, 160, and 320 μg/ml of CSC. In the control group, CSC was not added to the culture media during the development of 2PN embryos. In addition, the effects of 20 and 80 μg/ml of CSC on genes involved in pluripotency and apoptosis, and also, the aryl hydrocarbon receptor gene was assessed in the blastocysts. Our results showed that CSC had an adverse impact on the viability of mouse embryos at the concesntrations of 80, 160, and 320 μg/ml compared to the control group (P&lt;0.05). In contrast, it had positive effects on the viability of mouse embryos at the concesntrations of 20 and 40 μg/ml compared to the control group (P&lt;0.05). The 20 and 80 μg/ml concentrations of CSC increased the expressions of pluripotency, apoptotic, and the aryl hydrocarbon receptor genes in the blastocyst embryo stage compared to the control group (P&lt;0.05). It can be concluded that concesntrations higher than 40 μg/ml of cigarette smoke condensate have an adverse effect on mouse embryo development in the preimplantation stages. Also, 20 and 80 μg/ml concentrations of cigarette smoke condensate have a significant effect on the expression of pluripotency, apoptotic, and the aryl hydrocarbon receptor genes in the blastocyst embryo stage compared to the control group.</p> fardin fathi Copyright (c) https://www.excli.de/index.php/excli/article/view/3440