Anti-inflammatory activity of hydrosols from Tetragonia tetragonoides in LPS-induced RAW 264.7 cells

Authors

  • Eun-Yi Ko Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea
  • Su-Hyeon Cho Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea
  • Kyungpil Kang BKSU Lnc. Jeju , 63243, Republic of Korea
  • Gibeom Kim BKSU Lnc. Jeju , 63243, Republic of Korea
  • Ji-Hyeok Lee Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea
  • You-Jin Jeon Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea
  • Daekyung Kim Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea
  • Ginnae Ahn Department of Marine Bio-food science, College of Fisheries and Ocean Sciences, Chonnam National University, 500-749, Republic of Korea
  • Kil-Nam Kim Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Republic of Korea

DOI:

https://doi.org/10.17179/excli2017-121

Keywords:

Tetragonia tetragonoides, hydrosols, anti-inflammatory, NF-kappaB, MAPKs

Abstract

The present study was performed to investigate the anti-inflammatory activity of Tetragonia tetragonoides hydrosols (TTH) and its underlying mechanism in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Gas chromatography (GC) coupled with mass spectrometry and retention index calculations showed that TTH were mainly composed of tetratetracontane (29.5 %), nonacosane (27.6 %), and oleamide (17.1 %). TTH significantly decreased the production of nitric oxide (NO), prostaglandin E2 (PGE2), interleukin (IL)-6, and IL-1β in LPS-stimulated RAW 264.7 cells. Consistent with these observations, TTH treatment decreased the protein expression levels of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). The molecular mechanism of its anti-inflammatory activity was found to be associated with inhibition of nuclear factor-kappa B (NF-κB) phosphorylation and nuclear translocation of NF-κB 65. Furthermore, TTH markedly suppressed the LPS-induced phosphorylation of mitogen-activated protein kinases (MAPKs). Taken together, these data indicate that TTH exerts an anti-inflammatory activity by inhibiting the NF-κB and MAPK signaling pathways in LPS-stimulated RAW 264.7 cells.

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Published

2017-04-18

How to Cite

Ko, E.-Y., Cho, S.-H., Kang, K., Kim, G., Lee, J.-H., Jeon, Y.-J., … Kim, K.-N. (2017). Anti-inflammatory activity of hydrosols from Tetragonia tetragonoides in LPS-induced RAW 264.7 cells. EXCLI Journal, 16, 521–530. https://doi.org/10.17179/excli2017-121

Issue

Vol. 16 (2017)

Section

Original articles

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