Pinus Roxburghii essential oil anticancer activity and chemical composition evaluation

Authors

  • Arfaa Sajid Government College Women University Faisalabad, Faisalabad, Pakistan
  • Qaisar Manzoor Department of Chemistry, The University of Lahore, Lahore, Pakistan
  • Munawar Iqbal Department of Chemistry, The University of Lahore, Lahore, Pakistan
  • Amit Kumar Tyagi Cytokine Research Laboratory, Department of Experimental Therapeutics, The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA
  • Raja Adil Sarfraz Department of Chemistry, University of Agriculture, Faisalabad, Pakistan
  • Anam Sajid Department of Chemistry, University of Punjab, Lahore, Pakistan

DOI:

https://doi.org/10.17179/excli2016-670

Keywords:

Pinus roxburghii, anticancer agent, essential oil, NF-kappaB, MTT assay

Abstract

The present study was conducted to appraise the anticancer activity of Pinus roxburghii essential oil along with chemical composition evaluation. MTT assay revealed cytotoxicity induction in colon, leukemia, multiple myeloma, pancreatic, head and neck and lung cancer cells exposed to essential oil. Cancer cell death was also observed through live/dead cell viability assay and FACS analysis. Apoptosis induced by essential oil was confirmed by cleavage of PARP and caspase-3 that suppressed the colony-forming ability of tumor cells and 50 % inhibition occurred at a dose of 25 μg/mL. Moreover, essential oil inhibited the activation of inflammatory transcription factor NF-κB and inhibited expression of NF-κB regulated gene products linked to cell survival (survivin, c-FLIP, Bcl-2, Bcl-xL, c-Myc, c-IAP2), proliferation (Cyclin D1) and metastasis (MMP-9). P. roxburghii essential oil has considerable anticancer activity and could be used as anticancer agent, which needs further investigation to identify and purify the bioactive compounds followed by in vivo studies.

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Published

2018-03-12

How to Cite

Sajid, A., Manzoor, Q., Iqbal, M., Tyagi, A. K., Sarfraz, R. A., & Sajid, A. (2018). Pinus Roxburghii essential oil anticancer activity and chemical composition evaluation. EXCLI Journal, 17, 233–245. https://doi.org/10.17179/excli2016-670

Issue

Vol. 17 (2018)

Section

Original articles

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