Evaluation of adenosine deaminase (ADA) isoenzymes activity and tumor necrosis factor-α (TNFα) concentration in chronic heart failure

Authors

  • IrajI Khodadadi Department of Biochemistry and Nutrition, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
  • Mohammad Saleh Vahedi Kurdistan University of Medical Sciences, Sanandaj, Iran, Department of Anesthesia, Faculty of Para Medicine
  • Mohammad Abdi Kurdistan University of Medical Sciences, Sanandaj, Iran, Department of Pathology and Medical Laboratory Sciences, Faculty of Para Medicine
  • Nasrin Daneshkhah Kurdistan University of Medical Sciences, Sanandaj, Iran, Department of Anesthesia, Faculty of Para Medicine
  • Rezgar Rahbari Kurdistan University of Medical Sciences, Sanandaj, Iran, Department of Pathology and Medical Laboratory Sciences, Faculty of Para Medicine
  • Shahoo Menbari Kurdistan University of Medical Sciences, Sanandaj, Iran, Department of Pathology and Medical Laboratory Sciences, Faculty of Para Medicine
  • Dara Ahmadi Kurdistan University of Medical Sciences, Sanandaj, Iran, Department of Internal Medicine, Faculty of Medicine
  • Abbas Ahmadi Kurdistan University of Medical Sciences, Sanandaj, Iran, Departments of Hematology, Faculty of Medicine
  • Fariba Lahoorpour Kurdistan University of Medical Sciences, Sanandaj, Iran, Department of Pathology and Medical Laboratory Sciences, Faculty of Para Medicine
  • Mohammad Saied Hakhamaneshi Kurdistan University of Medical Sciences, Sanandaj, Iran, Department of Biochemistry and Nutrition, Faculty of Medicine
  • Saman Javid Department of Biochemistry and Nutrition, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
  • Bahram Nikkhoo Kurdistan University of Medical Sciences, Sanandaj, Iran, Department of Pathology and Medical Laboratory Sciences, Faculty of Para Medicine

Keywords:

Adenosine deaminase, heart failure, isoenzymes, tumor necrosis factor-alpha

Abstract

Introduction: Chronic heart failure (CHF) has recently been considered as an inflammatory disease. Enhanced production of tumor necrosis factor-α (TNFα) in CHF patients has been proved. To compensate deleterious effects of TNα, the concentration of adenosine is increased in CHF. However, concurrent determination of serum TNFα and enzymatic activities of ADA and its ADA1 and ADA2 isoenzymes, as the main regulators of adenosine concentration, has not yet been carried out.

Materials and Methods: Blood samples were collected from 52 CHF patients and 55 healthy controls. Laboratory routine tests were performed, and after determining the concentration of TNFα, total ADA (tADA) as well as ADA1 and ADA2 isoenzyme activities were measured.

Results: Mean concentration of TNFα increased over 2 fold in CHF patients (12.54 ± 11.69 pg/ml compared with 6.0 ± 6.58 pg/ml in controls). The highest level of TNFα was observed in patients with the final stage of the disease (NHYA IV subgroup), according to the New York Heart Association classification. tADA activity was significantly lower in CHF patients compared with controls (19.29 ± 9.73 and 24.3 ± 6.01 U/L, respectively). ADA2 activity markedly decreased in CHF patients and showed a direct correlation with tADA (r = 0.641, P = 0001). In addition, the lowest levels of tADA and ADA2 activities were observed in patients from the 4th quartile of NYHA classification.

Conclusion: Adenosine deaminase activity is reduced in CHF patients to give rise to the concentration of adenosine, thereby attenuating pathologic consequences of CHF. Therefore, it is concluded that ADA activity is of paramount importance in pathophysiology of heart failure and might be used for diagnostic purposes or treatment targets.

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Published

2014-02-03

How to Cite

Khodadadi, I., Vahedi, M. S., Abdi, M., Daneshkhah, N., Rahbari, R., Menbari, S., … Nikkhoo, B. (2014). Evaluation of adenosine deaminase (ADA) isoenzymes activity and tumor necrosis factor-α (TNFα) concentration in chronic heart failure. EXCLI Journal, 13, 58–66. Retrieved from https://www.excli.de/index.php/excli/article/view/682

Issue

Vol. 13 (2014)

Section

Original articles

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